We narrowed to 11,916 results for: loxP
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Plasmid#136236PurposeExpressing catalytically inactive human Argonaute-2 in insect cellsDepositorInserthuman Argonaute-2 (AGO2 Human)
TagsSterp SumoExpressionInsectMutationD597NPromoterpolyhedrinAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-DIO-mCherry-nega miR
Plasmid#137732PurposeLentivirus vector for control miR in a Cre-dependent mannerDepositorInsertcontrol microRNA
UseLentiviralTagsmChherryExpressionMammalianAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-lox-mCherryNLS-pA-lox-4493NES
Plasmid#138374PurposeBooster-PKA for the generation of miceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertlox-mCherry-lox-Booster-PKA
ExpressionMammalianPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40
Plasmid#105982PurposeThis AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.DepositorInsertFLEX-FAPdL5-POST-T2A-dTomato.FLEX
UseAAV and Cre/LoxTagsFAPdL5-POST, T2A-dTomato, and mycExpressionMammalianPromoterhuman synapsin 1Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MADR pDonor TagBFP2-V5-HRAS G12VWPRE
Plasmid#129420PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusionDepositorInsertTagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
UseMosaic analysis for dual recombinase-mediated cas…TagsTagBFP2-V5 tagMutationG12VPromoternone (3x polyA to mitigate episomal expression)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS6-TGFB2-STREP
Plasmid#128503PurposeExpresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.DepositorAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-UBE2G1 sg2R-P2A-Hygro
Plasmid#124299PurposeLentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertUBE2G1 (UBE2G1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLL3.7-EF-EYFP-YAP1_Y3F-PolyA
Plasmid#112288PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutation3 Tyrosines (341, 357 and 394 corresponding to ty…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR
Plasmid#113162PurposeA donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable markerDepositorInsertSpCas9
TagsFLAGExpressionMammalianMutationD10APromoterCAGAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptdTHC_PGKneoLox2DTA.2
Plasmid#112625PurposeH2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology armsDepositorInsertsUseCre/Lox and Mouse TargetingTagstdTomatoPromoterNo PromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-ERK:MEK
Plasmid#114291PurposeTET-inducible expression of ERK:MEK fusion proteinDepositorInsertrERK2 and hMEK1 proteins (Mapk1 Rat, Human)
UseLentiviralMutationrat ERK2 fused to human MEK1 with a linker in bet…Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCWXPGR-pTF-DVL2deltaDIX
Plasmid#114276PurposeTET-inducible expression of DVL2 protein with a deletion of its DIX-domainDepositorAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur
Plasmid#69052Purposelentiviral expression of IKZF2 variant 2 with HA tagDepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsHAExpressionMammalianMutationsplice variant 2PromoterUBCAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
44 pLIB_12HIS
Plasmid#160186PurposeN-term 3C cleavable 12xHis tag for insect cell expressionDepositorTypeEmpty backboneTags12xHisExpressionInsectPromoterpolyhydrinAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
46 pLIB_3C_C_HIS
Plasmid#160188PurposeC-term 3C cleavable His tag for insect cell expressionDepositorTypeEmpty backboneTags12xHisExpressionInsectPromoterpolyhydrinAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28GSTlinker-LIC
Plasmid#119970PurposeStructure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMaCTag-29
Plasmid#120008PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXP721
Plasmid#46055DepositorTypeEmpty backboneExpressionYeastPromoterGAL1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOTag4HM-crelox-PUR
Plasmid#24030DepositorTypeEmpty backboneUseCre/LoxTags3XHA and 3XMycAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only