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p3E-H2A_mCherry_SV40pA (JDW 967)

Plasmid

#224528

Purpose

A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Rabbit GFP Cav 2.2 pMT2

Plasmid

#58737

Purpose

expression of GFP tagged rabbit Cav2.2 in mammalian cells

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cav2.2 (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGMβ1

Plasmid

#216202

Purpose

Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

Depositor

Yasuko Sasaki

Article

Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

Type

Empty backbone

Use

Shuttle vector, conjugal plasmid, theta type rep…

Expression

Bacterial

Promoter

lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

Available Since

Jan. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Tags

8xHis and EGFP

Expression

Mammalian

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mKate2

Plasmid

#162624

Purpose

Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mKate2

Expression

Mammalian

Promoter

SP6

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Hemagglutinin-esterase

Plasmid

#168943

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Hemagglutinin-esterase (HE )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-hApg5(K130R)-HA

Plasmid

#22949

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Tags

3xHA

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-CAGGS (JDW 912)

Plasmid

#224472

Purpose

Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

CAGGS Promoter

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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