We narrowed to 3,409 results for: guide rna expression plasmid

1. dCas12Max-CBE

   Plasmid

   #195337

   Purpose

   vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized dCas12Max-CBE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N243R, E336R, D1049A

   Promoter

   CBh, CMV, hU6

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4254

   Plasmid

   #89050

   Purpose

   Expresses St chimeric gRNA and human codon-optimized StCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   St chimeric gRNA
   hStCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-dCas9-SSAP-MS2-BB_BbsI

   Plasmid

   #183826

   Purpose

   pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   U6-guideRNA-dCas9-T2A-EBFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBFC1207

   Plasmid

   #186694

   Purpose

   VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   Vc_2xBsaI_NT (Guide Stuffer) crRNA
   Tn7 transposon
   GmR+sfGFP VcDART Cargo
   SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

   Plasmid

   #223226

   Purpose

   guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

   Depositor

   Kyungjin Kim

   Article

   Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

   Insert

   Nr1d1 (Nr1d1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgGFP_3

   Plasmid

   #78165

   Purpose

   sgGFP

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-U6-sgPet-1-hSyn-mCherry-KASH

   Plasmid

   #223227

   Purpose

   guideRNA targeting the mouse Pet-1 (Fev)

   Depositor

   Kyungjin Kim

   Article

   Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

   Insert

   Fev (Fev Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgTP53_3

   Plasmid

   #78164

   Purpose

   sgTP53

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

9. Oxford Drosophila melanogaster Genome-wide CRISPR Library

   Pooled Library

   #64750

   Purpose

   A genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).

   Depositor

   Ji-Long Liu

   Article

   Bassett et al J Genet Genomics. 2015 Jun 20;42(6):301-

   Expression

   Insect

   Species

   Drosophila melanogaster, Streptococcus pyogenes, and Synthetic

   Use

   CRISPR

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only