We narrowed to 11,727 results for: EMB
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Plasmid#103126PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ5L8F8(Cas9 coding gene from Bacteroides fragilis (strain ATCC 25285 / NCTC 9343))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6WZS9
Plasmid#103103PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6WZS9(Cas9 coding gene from Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F4GDP9
Plasmid#103077PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF4GDP9(Cas9 coding gene from Alicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A8LN05
Plasmid#103083PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA8LN05(Cas9 coding gene from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1-HLx8
Plasmid#108139PurposeSAC1 with extended linkerDepositorInsertmCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1L(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mCFP
Plasmid#105142PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mCFP
Tags81nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mCFP
Plasmid#105139PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mCFP
Tags41nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter41nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GL2 shRNA
Plasmid#86083PurposeA lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.DepositorInsertcontrol shRNA
PromoterU6Available SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
mini-YFP-hDF-C2F-TM
Plasmid#72600Purposemini human dysferlin (from domain F to transmembrane ) tagged with YFPDepositorInsertdysferlin (DYSF Human)
TagsEYFPExpressionMammalianMutationfrom domain F to transmembrane domainPromoterCMVAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S710A
Plasmid#70149Purposemammalian expression of MKLP1 with an eGFP fusion and an S710A mutationDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET23 pEGFP-N2 (1174)
Plasmid#62037Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C/T180C
Plasmid#66182PurposeExpresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationR112C, T180CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C/L171C
Plasmid#66178PurposeExpresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationE105C, L171CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
TTMP-His-bio
Plasmid#52335PurposeExpresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertTTMP (C3orf52 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-puro/hFOXH1 shRNA3
Plasmid#59308PurposeKnockdown of human FOXH1DepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-Y20A
Plasmid#58460PurposeExpresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosine 20 to alaninePromoterCMV IEAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-deltaY20
Plasmid#58459PurposeExpresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosine 20 deletedPromoterCMV IEAvailable SinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SR
Plasmid#52142PurposeEncodes module 1 with amino acids 12S-16R for recognition of nt 8CDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
ExpressionBacterialMutationChanged 16Q to 16R in Module 1Available SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1NQ
Plasmid#52141PurposeEncodes module 1 with amino acids 12N-16Q for recognition of nt 8UDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
ExpressionBacterialMutationChanged 12S to 12N in Module 1Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only