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DH10beta F' DOT sbcC (donor strain)

Bacterial Strain

#12082

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Bacterial Resistance

Tetracycline

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
TP53 MITE Library

Pooled Library

#113569

Purpose

Pooled lentiviral plasmid library containing ~8,000 single amino acid substitution and nonsense mutant variants of p53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
pCF806-shABC2

Plasmid

#186714

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-AVI

Plasmid

#160475

Purpose

Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-NTD

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-AVI

Plasmid

#160477

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-SD1

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCF806-shABC1

Plasmid

#186713

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-AVI

Plasmid

#160474

Purpose

Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-S2P

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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HisGB1-Fusion + CBP TAZ1(340-439)

Plasmid

#173762

Purpose

Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

Depositor

Peter Wright

Article

Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

Inserts

CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
CBP (340-439) (Crebbp Mouse)

Tags

His6GB1 and thrombin

Expression

Bacterial

Mutation

fusion of CITED2 and Hif1a

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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HisGB1-FusionL21A + CBP TAZ1(340-439)

Plasmid

#173763

Purpose

Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

Depositor

Peter Wright

Article

Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

Inserts

CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
CBP (340-439) (Crebbp Mouse)

Tags

His6GB1 and thrombin

Expression

Bacterial

Mutation

fusion of CITED2 and Hif1a

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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HIsGB1-FusionL63A + CBP TAZ1(340-439)

Plasmid

#173764

Purpose

Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

Depositor

Peter Wright

Article

Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

Inserts

CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
CBP (340-439) (Crebbp Mouse)

Tags

His6GB1 and thrombin

Expression

Bacterial

Mutation

fusion of CITED2 and Hif1a

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L455RA475RG502R-AVI

Plasmid

#160480

Purpose

Expresses SARS-CoV-2 RBD-L455RA475RG502R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RA475RG502R

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine, Alanine 475 to A…

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI

Plasmid

#160479

Purpose

Expresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RG496R

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine and changed Glyci…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVI

Plasmid

#160478

Purpose

Expresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RA475R

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine and changed Alani…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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HR700_TP53Exon5mut

Pooled Library

#229137

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 5 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available Since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
HR700_TP53Exon6mut

Pooled Library

#229138

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 6 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available Since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
HR700_TP53Exon7mut

Pooled Library

#229139

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 7 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available Since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
HR700_TP53Exon8mut

Pooled Library

#229140

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 8 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available Since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

Plasmid

#209085

Purpose

Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

Plasmid

#209086

Purpose

Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Tags

AviTag

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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