We narrowed to 9,277 results for: Pol;

1. HUHe variant click editor (CE1) - pCMV-T7-Chimp17-nCas9-EcKlenow (JO1275)

   Plasmid

   #217792

   Purpose

   Variant CE1 construct with Chim HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Chimp17-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HUHe variant click editor (CE1) - pCMV-T7-GoCV-nCas9-EcKlenow (JO1277)

   Plasmid

   #217793

   Purpose

   Variant CE1 construct with GoCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   GoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. HUHe variant click editor (CE1) - pCMV-T7-SwCV-nCas9-EcKlenow (JO1270)

   Plasmid

   #217794

   Purpose

   Variant CE1 construct with SwCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   SwCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HUHe variant click editor (CE1) - pCMV-T7-RaCV-nCas9-EcKlenow (JO1268)

   Plasmid

   #217795

   Purpose

   Variant CE1 construct with RaCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   RaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HUHe variant click editor (CE1) - pCMV-T7-BDFV-nCas9-EcKlenow (JO1271)

   Plasmid

   #217785

   Purpose

   Variant CE1 construct with BDFV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   BDFV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HUHe variant click editor (CE1) - pCMV-T7-CoCV-nCas9-EcKlenow (JO1262)

   Plasmid

   #217786

   Purpose

   Variant CE1 construct with CoCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   CoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

   Plasmid

   #217787

   Purpose

   Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

   Plasmid

   #217788

   Purpose

   Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

   Plasmid

   #217789

   Purpose

   Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX-Sox17-p2A-Erg-t2A-GFP

    Plasmid

    #222496

    Purpose

    mouse fibroblast-to-endothelial cell direct reprogramming

    Depositor

    Li Qian

    Article

    Farber et al Nat Commun. 2024 May 16;15(1):4170. doi:

    Inserts

    Sox17 (Sox17 Mouse)
    Erg (Erg )
    EGFP
    

    Use

    Retroviral

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNK845

    Plasmid

    #219746

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal luciferase
    

    Use

    Luciferase and Synthetic Biology

    Mutation

    I3S, N4T, F11L, I63T, T99P, T192S, A199P

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF1953

    Plasmid

    #142897

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6

    Plasmid

    #212820

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-His6 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Tags

    CUbo(K63R)-6xHis

    Expression

    Mammalian

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT-TO-EGFR*-CUbo(K48R)-GFP

    Plasmid

    #212816

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R)-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Tags

    CUbo(K48R)-GFP

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP

    Plasmid

    #212817

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Tags

    CUbo(K63R)-GFP

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP

    Plasmid

    #212818

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Tags

    CUbo(K48R/K63R)-GFP

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PABPC1_544-636_pGEX6P1

    Plasmid

    #209789

    Purpose

    Bacterial expression of PABPC1 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al EMBO J. 2004 Jan 28;23(2):272-81. doi: 1

    Insert

    Mlle domain of PABPC1 (PABPC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 544-636 only

    Promoter

    tac

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-AN-DDK_WT RAD23A_delta aa 230-285 (delta XPC-binding domain)

    Plasmid

    #201464

    Purpose

    Expresses a mutant form of human RAD23A that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 230-285

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

    Plasmid

    #196879

    Purpose

    Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG416GALL-Bat-CoV-HKU9_3CL

    Plasmid

    #203478

    Purpose

    Expresses Bat-CoV HKU9 3CL protease from a GALL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    Bat-CoV HKU9 3CL protease (ORF1ab )
    

    Expression

    Yeast

    Promoter

    GALL

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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