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pRRL-GFPGO2(NGT)-PGK-Neo

Plasmid

#136892

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2(NGT)

Use

Lentiviral

Tags

2xNLS

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.178

Plasmid

#119645

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS3 Down Flank

Use

Synthetic Biology

Mutation

4 nucleotide insertion between nt 65 and 66 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.165

Plasmid

#119634

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS1 Up Flank

Use

Synthetic Biology

Mutation

1 nucleotide insertion between nt 21 and 22 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.176

Plasmid

#119644

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS2 Down Flank

Use

Synthetic Biology

Mutation

bp 302 T to C

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.175

Plasmid

#119643

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS2 Up Flank

Use

Synthetic Biology

Mutation

bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.174

Plasmid

#119641

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS1 Down Flank

Use

Synthetic Biology

Mutation

bp 658 G to A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.171

Plasmid

#119639

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS4 Up Flank

Use

Synthetic Biology

Mutation

A691T in NS4 Up Flank

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.169

Plasmid

#119637

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS3 Up Flank

Use

Synthetic Biology

Mutation

bp 551 T to C, bp 554 T to C

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.018

Plasmid

#119622

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

dCas9+deg-tag

Use

Synthetic Biology

Mutation

bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.054

Plasmid

#119572

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

PpetE

Use

Synthetic Biology

Mutation

RBS* added (22 bp) upstream of ATG. Deletion of n…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.055

Plasmid

#119566

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

PisiAB

Use

Synthetic Biology

Mutation

bp 391 G to A, RBS* added (22 bp) upstream of ATG…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.057

Plasmid

#119565

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ParsB

Use

Synthetic Biology

Mutation

bp 117 G to A, RBS* added (22 bp) upstream of ATG.

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAT.334

Plasmid

#119556

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.079

Plasmid

#119667

Purpose

Level 0 part. Terminator for the psbA2 gene Synechocystis sp. PCC 6803.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TpsbA2

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.066

Plasmid

#119666

Purpose

Level 0 part. Terminator from pheA gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TpheA-1

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.080

Plasmid

#119665

Purpose

Level 0 part. Terminator for the cpcG1 gene Synechocystis sp. PCC 6803.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TcpcG1

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.063

Plasmid

#119664

Purpose

Level 0 part. Terminator derived from Biobricks plasmid pSB1AK3.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

pSB1AK3 terminator

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.074

Plasmid

#119663

Purpose

Level 0 part. Terminator from Cysteine synthase A gene (cysK) from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120048902

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.071

Plasmid

#119662

Purpose

Level 0 part. Terminator from tatABCD operon from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120030798

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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