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p5343_UC57

Plasmid

#126645

Purpose

environmental plasmid p5343 cloned into pUC57

Depositor

Aindrila Mukhopadhyay

Article

Kothari et al mSystems. 2019 Dec 17;4(6). pii: 4/6/e00

Insert

Environmental plasmid p5343

Use

Synthetic Biology

Promoter

NA

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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UL91-2xStrep pCDNA4

Plasmid

#120375

Purpose

Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL91 (UL91 Human Cytomegalovirus (HHV-5))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSPneoSagRNAH

Plasmid

#123265

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNeoSagRNAH

Plasmid

#123266

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1259R

Plasmid

#118995

Purpose

mCherry (without start and stop codons) in pGFL1010

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

mCherry (without start and stop codons)

Use

Fungal expression

Promoter

promoter less

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1260

Plasmid

#116900

Purpose

eGFP:TubA (tubulin marker )

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

TubA promoter
TubA (ORF+3'UTR)

Use

Fungal expression (in magnaporthe oryzae)

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1260R

Plasmid

#116901

Purpose

mCherry:TubA (tubulin marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

TubA promoter
TubA (ORF+3'UTR)

Use

Fungal expression (in magnaporthe oryzae)

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSM002

Plasmid

#122936

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML043C (Marked)

Expression

Yeast

Mutation

YML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATAC

Promoter

[tetO2]4inPgal1

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSM001

Plasmid

#122935

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

[tetO2]4inPgal1

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEC005

Plasmid

#122939

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML071C (Marked)

Expression

Yeast

Mutation

YML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGC

Promoter

[tetO2]4inPgal1

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_207

Plasmid

#122938

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML070W (Marked)

Expression

Yeast

Mutation

YML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTA

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_243

Plasmid

#122940

Purpose

Expression of the marked mRNA under the control of insertional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

Pyml029w-tetO(2)

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSM004

Plasmid

#122937

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML065W (Marked)

Expression

Yeast

Mutation

YML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAAC

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_228

Plasmid

#122933

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR025C (Marked)

Expression

Yeast

Mutation

YBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTA

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_229

Plasmid

#122934

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR026C (Marked)

Expression

Yeast

Mutation

YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

Promoter

[tetO2]4inPgal1

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2NBAA

Plasmid

#107873

Purpose

Bacterial expression of FGF2 minimum export mutant-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-minimal export

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

C77A, C95A, K128Q, R129Q, K134Q

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pWT060b

Plasmid

#107904

Purpose

W2m.0-2 plasmid. Contains U6-sgRNA A (CCR5) and is part of a system for CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA A (CCR5) (CCR5 Human)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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Rab42b cds in pGem-T Easy

Plasmid

#80564

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab42b (rab42b Zebrafish)

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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