We narrowed to 9,277 results for: Pol;

1. pCAG-rox-inv[Talpha1-iCre-pA]-rox-mNeonGreen-hCentrin1

   Plasmid

   #196892

   Purpose

   Neuron-specific expression of human (h)Centrin fused to mNeonGreen. Used in combination with Talpha1-Dre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1

   Plasmid

   #196891

   Purpose

   Neuron-specific expression of human (h)Centrin fused to 2xHA-tags

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-Nedd1-mOrange2-rCM1

   Plasmid

   #196861

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-Nedd1-mOrange2-F75A

   Plasmid

   #196862

   Purpose

   Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-F75A (Nedd1 Rat)
   

   Expression

   Mammalian

   Mutation

   F75A in the CM1 domain

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBactin-Akna-mOrange2

   Plasmid

   #196865

   Purpose

   Expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPL3-hRPH3A_c.444Tmut

   Plasmid

   #190477

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Expression

   Mammalian

   Mutation

   c.444G>T

   Promoter

   SV40

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSPL3-hRPH3A_c.1853Awt

   Plasmid

   #190478

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187611

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187612

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain (R163A) with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Arg163Ala

   Promoter

   EF1A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT-FLAG-CBPΔN

    Plasmid

    #182840

    Purpose

    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    

    Tags

    FLAG tag

    Expression

    Insect

    Promoter

    MT

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX2T-TEV CHMP1B 186-199

    Plasmid

    #108285

    Purpose

    Expresses residues 186-199 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-283

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 186-199

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX2T-TEV CHMP1B 144-178

    Plasmid

    #108287

    Purpose

    Expresses residues 144-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-281

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 144-178

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX2T-TEV CHMP1B 4-199 L188A/L192A

    Plasmid

    #108289

    Purpose

    Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed L 188 to A, changed L 192 to A

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX2T-TEV CHMP1B 4-199 L192A/L195A

    Plasmid

    #108291

    Purpose

    Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed L 192 to A, changed L 195 to A

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1delZn3-L713F

    Plasmid

    #173934

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCJ205

    Plasmid

    #162676

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224A, C529A; codon optimized for expression in E…

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCJ198

    Plasmid

    #162671

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    F495I; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCJ196

    Plasmid

    #162669

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S225A; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCJ204

    Plasmid

    #162677

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224H, C529F; codon optimized for expression in E…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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