We narrowed to 18,122 results for: multi
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Plasmid#223509PurposePylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsExpressionMammalianPromoterCMV, SP6 and U6Available SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas3-Km
Plasmid#207999PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mTagBFP2-WPRE
Plasmid#193339PurposeAAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseAAVExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (golgi) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS
Plasmid#129532PurposeSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4DepositorInsertscodon-optimized AcrIIA4
sgRNA_Rosa26
UseAAVTagsFLAG/NLSPromoterU6 and cytomegalovirus-enhancer chicken β-actin p…Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
ScFv-2ERT2-VPH
Plasmid#120556PurposeEncode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1DepositorInsertscFvGCN4, GB1, ERT2, VP64, P65 and HSF1
UseLentiviralExpressionMammalianPromoterhPGKAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR348
Plasmid#124713PurposeExpression of narX under PtacDepositorUseSynthetic BiologyExpressionBacterialMutationS193* - LacI is truncated and likely deactivated.…Available SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pActinin-miRFP670nano
Plasmid#127432PurposeAlpha actinin labeling with near-infrared fluorescent proteinDepositorInsertAlpha-actinin-miRFP670nano
TagsmiRFP670nanoExpressionMammalianPromoterCMVAvailable SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1584
Plasmid#121055PurposeYPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertYPET^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized YPET, and auxi…ExpressionWormAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104119PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseAdenoviralExpressionMammalianPromoterhuman synapsin IAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-MsCHI
Plasmid#73394PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
MsCHI
ExpressionBacterialPromoterT7Available SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-H2B-EGFP-P2A-N2c(G)
Plasmid#73482PurposeCloning vector containing N2c Glycoprotein and histone GFPDepositorInsertH2B-EGFP-P2A-N2c(G)
ExpressionMammalianAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
XW17
Plasmid#65835PurposePunc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
TagsmCherryExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185H/C280S in pET15b
Plasmid#61672PurposeHIV-1 integrase F185H/C280S in pET-15bDepositorInsertHIV-1 integrase F185H/C280S
UseSynthetic BiologyTagsHisPromoterT7 promotorAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
BAT-lux
Plasmid#20890DepositorInsert7xTCF binding sites, Siamois minimal promoter
UseLuciferaseExpressionMammalianMutation7 multimerized TCF-binding elements fused upstrea…Available SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV1)
Viral Prep#198516-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF-1aTagsmScarletAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-FingR-PSD95-mGL
Plasmid#217533PurposeGenetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivoDepositorArticleInsertZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)
UseAAVTagsmGreenLanternExpressionMammalianPromoterS5E2 enhancerAvailable SinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32 (MSW#497)
Plasmid#208068PurposeExpression of GFP-tagged hsRPA2 in human cellsDepositorAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
TagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only