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We narrowed to 18,348 results for: multi

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Showing: 6841 - 6860 of 18348 results

  2. pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47446
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5HD_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47446-plasmid-map-sequence-id-69438

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  6. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Publication
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  8. pAAVS1-CAG-hCAS9-neo

    Plasmid
    #166026
    Purpose
    AAVS1 targeting vector carrying CAG-SpCas9-neo
    Depositor
    Kosuke Yusa
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAGCas9IRESneo
    Expression
    Mammalian
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166026-plasmid-map-sequence-id-328056

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  12. pGAPTrap-mCherry-IRESMygro

    Plasmid
    #82505
    Purpose
    Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.
    Depositor
    Ed Stanley
    Publication
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-IRESMygro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82505-plasmid-map-sequence-id-156572

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  14. pAC-ZETA

    Plasmid
    #53316
    Purpose
    Contains Erwinia herbicola Eho10 genes crtE and crtB, together with the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene at the N terminus. Produces zeta-carotene in E. coli.
    Depositor
    Francis X Cunningham Jr
    Publication
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    pds
    Use
    Low copy number bacterial cloning vector
    Promoter
    lazZ
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    53316-plasmid-map-sequence-id-83035

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  16. FNL Combinatorial Cloning Platform

    Plasmid kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning type
    MultiSite Gateway
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV_hSyn-PdCO-mScarlet-WPRE (AAV5)

    Viral prep
    #198510-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Publication
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. YFP(159-238)-gamma-2 in pcDNAI/Amp

    Plasmid
    #54472
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(159-238)-gamma -2 (GNG2 Human, Aequorea victoria)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-2 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54472-plasmid-map-sequence-id-115042

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  19. GRM2-DuET

    Plasmid
    #213301
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM2 (GRM2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213301-plasmid-map-sequence-id-430907

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Showing: 6841 - 6860 of 18348 results