We narrowed to 18,495 results for: REV

1. pET PI-SceI C-His K301A

   Plasmid

   #195611

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   A822G, A823C, A824T

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193318

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Tags

   4MYC

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193317

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Tags

   4FLAG

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET PI-SceI C-His

   Plasmid

   #195610

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

   Promoter

   T7

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-eGFP-TAX1BP1 Q770A E774K

   Plasmid

   #175770

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Q770A E774K

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Lentiviral

   Mutation

   Q770A E774K

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcrVIB1-cloDF-amp

   Plasmid

   #184843

   Purpose

   Expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXD70LacZ7-DadR/DadS-Pdadh-LacZ

   Plasmid

   #191622

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadS

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXD70LacZ8-CadR-Pcadh-LacZ

   Plasmid

   #191623

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXD70LacZ9.1-HcdR-Phcdh-LacZ

   Plasmid

   #191629

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdR

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXD70Tet(DadRmt4)-DadR(linker)

    Plasmid

    #191639

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Expression

    Bacterial

    Mutation

    Linker region sequence shuffled

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR

    Plasmid

    #191640

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)

    Plasmid

    #191641

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔDBD

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)

    Plasmid

    #191642

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔTM

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

    Plasmid

    #191643

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Linker region sequence shuffled

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXD70Tet(DadH)-Pdadh-DadhABC(A2)

    Plasmid

    #191644

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBSKΔB-24xopto-TetO-no01

    Plasmid

    #174887

    Purpose

    Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    24xopto-TetO
    

    Use

    Southern blot probe

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 sf:bleach.1

    Plasmid

    #191929

    Purpose

    Encodes a GFP design, sf:bleach.1

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:bleach.1
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T108L Y145M T167V L220V

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28 mid.13

    Plasmid

    #191915

    Purpose

    Encodes a GFP design, mid.13

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.13
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72V Y145S

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28 mid.6

    Plasmid

    #191899

    Purpose

    Encodes a GFP design, mid.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.6
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V68M S72T Y145M V224I

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 mid.5

    Plasmid

    #191896

    Purpose

    Encodes a GFP design, mid.5

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.5
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Y145M T167V H181Y L220V V224I

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28 mid.4

    Plasmid

    #191895

    Purpose

    Encodes a GFP design, mid.4

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.4
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V68A S72A Y145M L220V

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Linked Free NES (M)

    Plasmid

    #182436

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    FPPS(M)-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Coexpressed Free NES (M)

    Plasmid

    #182437

    Purpose

    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    FPPS(M)
    NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10 and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28 sf:bleach.3

    Plasmid

    #191925

    Purpose

    Encodes a GFP design, sf:bleach.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:bleach.3
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A V112I Y145M T167V H181I V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28 bleach.3

    Plasmid

    #191914

    Purpose

    Encodes a GFP design, bleach.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.3
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A V112I Y145M T167V H181I V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28 mid.12

    Plasmid

    #191913

    Purpose

    Encodes a GFP design, mid.12

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.12
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72V Y145M T167V H181I V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28 em-blue-shift.2

    Plasmid

    #191901

    Purpose

    Encodes a GFP design, em-blue-shift.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    em-blue-shift.2
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72A T108I Y145F V150I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28 acid.5

    Plasmid

    #191903

    Purpose

    Encodes a GFP design, acid.5

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.5
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145M T167V H181V

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28 acid.4

    Plasmid

    #191898

    Purpose

    Encodes a GFP design, acid.4

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.4
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V61L Q69L Y145M L220V

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28 acid.3

    Plasmid

    #191897

    Purpose

    Encodes a GFP design, acid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.3
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69L S72A T108V Y145M V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge