We narrowed to 11,576 results for: Vars;

1. pAG8Ha-EWS_RRM-RGG(359-513)

   Plasmid

   #188046

   Purpose

   Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-358, 514-656

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEmal10H-MBP-EWSR1-449-513

   Plasmid

   #199439

   Purpose

   Expresses RG2 (the second RG-rich region, 449-513) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-448, 514-656

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. macroH2A2-GFP (pc2191)

   Plasmid

   #223709

   Purpose

   Expression of macroH2A2 tagged N-terminal to GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. macroH2A1.2-F192V-GFP (pc5115)

   Plasmid

   #223438

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.2-F192V (Macroh2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of phenylalanine 192 to valine (F192V)

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Control-TP53-LRG-GFP

   Plasmid

   #225874

   Purpose

   Guides targeting P53 and a control sequence

   Depositor

   Janai Carr-Ascher

   Article

   Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

   Insert

   Control and TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Control-RB1-LRG-GFP

   Plasmid

   #225875

   Purpose

   Guides targeting RB1 and a control sequence

   Depositor

   Janai Carr-Ascher

   Article

   Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

   Insert

   Control and RB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

   Plasmid

   #220614

   Purpose

   Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   3x HA; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnillin homology domain

   Plasmid

   #187277

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Anillin homology domain

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   deltaAnillin homology domain

   Promoter

   U6, CMV

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458-3xHA-dFnCas9

   Plasmid

   #201954

   Purpose

   Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dFnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A and H969A on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-His6-en1dFnCas9GFP

    Plasmid

    #201950

    Purpose

    Expression of en1dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en1dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1369R on FnCas9

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-His6-en15dFnCas9GFP

    Plasmid

    #201951

    Purpose

    Expression of en15dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en15dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1603H on FnCas9

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX458-3xHA-dSpCas9

    Plasmid

    #201953

    Purpose

    Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dSpCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A on SpCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

    Plasmid

    #209197

    Purpose

    Mutagenesis of Faah

    Depositor

    Larry Zweifel

    Article

    Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

    Insert

    Faah (Faah Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE FL NAP1 STOP C' EGFP IRES Blast

    Plasmid

    #179102

    Purpose

    Expresses untagged full length NAP1

    Depositor

    Alicia Pickrell

    Article

    Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

    Insert

    Nak associated protein 1 (AZI2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-N'-FLAG-HA-NAP1S318A

    Plasmid

    #195410

    Purpose

    Expresses FLAG-HA tagged human NAP1 with S318A point mutation

    Depositor

    Alicia Pickrell

    Article

    Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

    Insert

    Nak associated protein 1 (AZI2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Serine 318 turned to Alanine

    Promoter

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ERAP1-rs27895(T)

    Plasmid

    #206143

    Purpose

    Expresses the rs27895-T allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mutation

    G346 changed to D346

    Promoter

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2/shCol1a1/Scarlet_Seq1.3

    Plasmid

    #206137

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAVss-U6-sgHTT51-7sk-Cas9

    Plasmid

    #190901

    Purpose

    AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA (Htt Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and 7sk

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1

    Plasmid

    #190902

    Purpose

    AAV vector expressing thew GFP reporter gene and human sgHTT

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    GFP and sgHTT (HTT Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-cT3G-cERP2-LHX9

    Plasmid

    #192921

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of LHX9

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    LHX9 (LHX9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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