We narrowed to 18,457 results for: multi

1. AKARdual

   Plasmid

   #99869

   Purpose

   Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   AKAR4
   

   Tags

   LSSmOrange and mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dpYPQ230-D156R-ABE5

   Plasmid

   #195371

   Purpose

   dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   ecTadA8e-(GGGGS)x8-dLbCas12a-D156R
   

   Use

   CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

   Tags

   5' and 3' NLS

   Expression

   Plant

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ePPEmax*

   Plasmid

   #205244

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Lyn-PlcR(K30/32L)

   Plasmid

   #181831

   Purpose

   Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-PlcR (K30L,K32L)
   

   Tags

   ECFP, EYFP, and N-terminal targeting sequence fro…

   Expression

   Mammalian

   Mutation

   Lysines 30 and 32 in PH domain mutated to leucine.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pemiRFP703-tubulin

   Plasmid

   #136564

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP703-tubulin
   

   Tags

   emiRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6_(GLuc)_INT[3xMS2_SL]

   Plasmid

   #68426

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three MS2 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.13/AUG-FFLuc-3XFLAG

   Plasmid

   #127333

   Purpose

   Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   Firefly luciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CV40

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR221-NR1H3

   Plasmid

   #79514

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR Alpha (NR1H3 Human)
   

   Use

   Multisite gateway entry clone

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1-CaMKKbeta

    Plasmid

    #32453

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Barcode backbone

    Plasmid

    #158665

    Purpose

    This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    hcRED

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMuLE ENTR tdTomato L5-L2

    Plasmid

    #62185

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    tdTomato
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti_CMV:Flag-OgNLuc

    Plasmid

    #208851

    Purpose

    Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    OgNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-Ago1

    Plasmid

    #21534

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid

    #118471

    Purpose

    Negative-control mutant for blueAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NLS-dCas9-HA-NLS-GFP-VP16

    Plasmid

    #183923

    Purpose

    VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP-VP16
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ3 SHP2 WT

    Plasmid

    #8317

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Expression

    Mammalian

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJOG1368

    Plasmid

    #167301

    Purpose

    Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    FCY-UPP fusion gene
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti_CMV:Flag-GeNL

    Plasmid

    #208841

    Purpose

    Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    GeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Promoter

    GAL4-responsive UAS hsp70

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge