We narrowed to 9,186 results for: mel

1. Kras (G12V)-pcw107

   Plasmid

   #64602

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   G12V

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Notch1 intracellular domain-pcw107-V5

   Plasmid

   #64622

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   NOTCH1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   intracellular domain *

   Promoter

   PGK

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLKO.1 Sox2 3H b

   Plasmid

   #26352

   Depositor

   Matthew Meyerson

   Article

   Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

   Insert

   Sox2 shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-Phi3-AURKB-PGK-mCherry

   Plasmid

   #153316

   Purpose

   Expresses AURKB and mCherry in mammalian cells.

   Depositor

   Robert Judson-Torres

   Article

   McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

   Insert

   AURKB (AURKB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AR-V7-pcw107

   Plasmid

   #64635

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   AR (transcript variant 1, splice isoform) (AR Human)
   

   Use

   Lentiviral

   Mutation

   splice isoform*

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52379

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4
   

   Expression

   Mammalian

   Promoter

   OCT4

   Available Since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-AlkB

   Plasmid

   #228218

   Purpose

   Express wild type AlkB protein in E. coli BL21(DE3)

   Depositor

   Junchao Shi

   Article

   Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

   Insert

   Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   codon optimized

   Promoter

   T7

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. beta-catenin (S33A, S37A, T41A, S45A)-pcw107

   Plasmid

   #64612

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Mutation

   S33A, S37A, T41A, S45A

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. beta-catenin (S33Y)-pcw107-V5

   Plasmid

   #64616

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   S33Y

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

10. IKKalpha (S176E,S180E)-pcw107-V5

    Plasmid

    #64608

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S176E, S180E

    Promoter

    PGK

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221-GATA4

    Plasmid

    #101417

    Purpose

    Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. NES-EGFP-PH-BTKx2

    Plasmid

    #183652

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 2-174 and 2-170

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1α1.1-ChrimsonR-GFP

    Plasmid

    #122063

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV2

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1a（1.1kb short version)

    Available Since

    March 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AR-V7-pcw107-V5

    Plasmid

    #64636

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AR (transcript variant 1, splice isoform) (AR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    splice isoform *

    Promoter

    PGK

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGL3Basic_ME.1/ApoEpromoter

    Plasmid

    #51435

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    ME.1 enhancer is fused upstream of ApoE promoter …

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pNTK human MBD3 PGK-Neo-pA flox

    Plasmid

    #52362

    Purpose

    Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    MBD3 (MBD3 Human)
    

    Use

    Targeting

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. NES-mCherry-PH-BTKx2

    Plasmid

    #183654

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Tags

    X. laevis map2k1.L(32-44)-mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-174 and 2-170

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Notch1 intracellular domain-pcw107

    Plasmid

    #64621

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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