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169,450 results

Showing: 10351 - 10380 of 169450 results

  5. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  7. pSLIK sh human Rb 1534 hyg

    Plasmid
    #31500
    Depositor
    Julien Sage
    Insert
    RB shRNA (RB1 Human)
    Use
    Lentiviral and RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    The target sequence for shRB 1534 is GAACGATTATCC…
    Available Since
    Aug. 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    31500-plasmid-map-sequence-id-14427

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  12. pGWB501NL3F10H

    Plasmid
    #141286
    Purpose
    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Type
    Empty backbone
    Use
    Luciferase; Gateway
    Tags
    FLAG (x3), HIS (x10), and NLUC
    Expression
    Plant
    Available Since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    141286-plasmid-map-sequence-id-324828

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  14. DR274

    Plasmid
    #42250
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
    Depositor
    Keith Joung
    Article
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR; Zebrafish expression
    Expression
    Worm
    Promoter
    T7
    Available Since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    42250-plasmid-map-sequence-id-254852

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  15. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

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  16. TFORF3119

    Plasmid
    #144595
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DDIT3 (DDIT3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144595-plasmid-map-sequence-id-404295

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  17. pD649-HAsp-KIR2DL5A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156643
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    KIR2DL5A (KIR2DL5A Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156643-plasmid-map-sequence-id-430447

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  19. CAG-eGFP-Z1

    Plasmid
    #135046
    Purpose
    Enhanced Green fluorescent protein under constitutive CAG Promoter for mammalian expression in minimal backbone plasmid
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CAG Promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135046-plasmid-map-sequence-id-269409

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  25. ER-M-TAG (pGS555)

    Plasmid
    #160040
    Purpose
    pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    MMGT1-mScarlet-3xHA
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160040-plasmid-map-sequence-id-323176

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Showing: 10351 - 10380 of 169450 results