We narrowed to 7,716 results for: ALP

1. pmCherry-3xNLS-OptoJNKi

   Plasmid

   #89740

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKTop::tse5-CT (1169-1281)

   Plasmid

   #192955

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1281)
   

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Available Since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

   Plasmid

   #186417

   Purpose

   Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

   Depositor

   Mary Kay Lobo

   Article

   Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

   Insert

   Early growth response 3 (Egr3 Mouse)
   

   Use

   AAV

   Tags

   EYFP

   Promoter

   EF-1-alpha

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGB 35s:dCas:EDLL:tNos (GB1190)

   Plasmid

   #75404

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas:EDLL
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-DCRD-YFP

   Plasmid

   #87450

   Purpose

   rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

   Depositor

   Xiaodong Liu

   Article

   Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

   Insert

   DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
   

   Tags

   6xHis-T7, FKBP, and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKCAG-mCherry-OptoJNKi

   Plasmid

   #89738

   Purpose

   Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

   Plasmid

   #186967

   Purpose

   Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   DNMT3L (DNMT3L Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   rTetR fusion

   Expression

   Mammalian

   Promoter

   EF-1alpha promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-NES-OptoJNKi

   Plasmid

   #89739

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKCAG-H2BmCherry-OptoJNKi

   Plasmid

   #89741

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Optogenetics

   Tags

   Histone2B and mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBullet-cg-c

    Plasmid

    #53070

    Purpose

    destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

    Plasmid

    #181842

    Purpose

    EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains only residues 12-112 of PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRIM24 gRNA (BRDN0001147238)

    Plasmid

    #77889

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKN1 gRNA (BRDN0001162227)

    Plasmid

    #76276

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-GINA3

    Plasmid

    #74980

    Purpose

    Expresses His tagged GINA3 protein in bacteria.

    Depositor

    Pradipta Ghosh

    Article

    Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

    Insert

    Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CFP-PCRD-FRB

    Plasmid

    #87449

    Purpose

    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    

    Tags

    6xHis-T7, CFP, and FRB

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-45b-RUNX2(+10A)-IDR-mCherry

    Plasmid

    #145287

    Purpose

    Bacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(+10A)-IDR (RUNX2 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGB 35s:dCas:VP64:tNos (GB1189)

    Plasmid

    #75403

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas:VP64
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. CD8a-cNLS-EGFP

    Plasmid

    #86052

    Purpose

    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CD8a luminal and transmembrane along with two SV4…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PRKAA1 gRNA (BRDN0001147273)

    Plasmid

    #76254

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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