We narrowed to 57,954 results for: NAM

1. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

   Plasmid

   #52898

   Purpose

   YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Ypd*1-L-IFP-F[2]
   

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Changed Tryptophane 80 to Alanine

   Promoter

   GAL1

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGMCK-0X750-luxAB-DAS+4

   Plasmid

   #49517

   Purpose

   Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   P750-luxAB-DAS+4
   

   Tags

   DAS+4 (AANDENYSENYADAS)

   Expression

   Bacterial

   Promoter

   P750

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET12a-AL-103 Y32F Y96F

   Plasmid

   #47092

   Depositor

   Marina Ramirez-Alvarado

   Article

   DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

   Insert

   AL-103 Y32F Y96F (IGKV3-20 Human)
   

   Tags

   ompT

   Expression

   Bacterial

   Mutation

   Y32F Y96F

   Promoter

   T7

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET15b/GgVcl 1-851 A50I mutation

   Plasmid

   #46174

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

   Insert

   Vcl 1-851 A50I (VCL Chicken)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-851 A50I mutation (talin binding domain mut…

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR SFFVp PHAkt Cerulean

   Plasmid

   #50837

   Purpose

   Fluorescent reporter for membrane PIP3 levels

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PHAkt (AKT1 )
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Cerulean

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLJR965

   Plasmid

   #115163

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR (deleted TetON) MtbCO
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6-CENP-A-mCherry

   Plasmid

   #89767

   Purpose

   Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CENP-A (CENPA Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TORCAR

   Plasmid

   #64927

   Purpose

   FRET-based biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR (EIF4EBP1 Synthetic, Human)
   

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5HT6-G-GECO1.0

   Plasmid

   #47499

   Purpose

   Fluorescent reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
   

   Tags

   G-GECO1.0

   Expression

   Mammalian

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti_dCas9-KRAB-MeCP2

    Plasmid

    #122205

    Purpose

    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Lentiviral

    Tags

    KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Cas9 (D10A, H840A)

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 HA-Sumo1 WT

    Plasmid

    #48966

    Purpose

    Expresses HA-Sumo1 WT in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid

    #84563

    Purpose

    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    

    Use

    Lentiviral

    Tags

    myc tag (9x)

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
    

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MIB2-FLAG

    Plasmid

    #37117

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. PLJR962

    Plasmid

    #115162

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA Flag ERbeta

    Plasmid

    #35562

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ1661-sgMUC4-E3(F+E)

    Plasmid

    #51025

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    optimized sgRNA (MUC4 Mouse, Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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