We narrowed to 18,457 results for: multi

1. pAAV-TRE-tdKatushka2-WPRE

   Plasmid

   #193344

   Purpose

   AAV-mediated expression of tdKatushka2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   tdKatushka2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE666

   Plasmid

   #153231

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IPU-mTagBFP2-Sec61B

   Plasmid

   #228136

   Purpose

   Expresses mTagBFP2-Sec61B in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   Sec61B (SEC61B Human)
   

   Use

   Retroviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKII-VARNAM2

   Plasmid

   #195527

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   CaMKII

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

   Plasmid

   #182012

   Purpose

   CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   CalR-HaloTag7-P30-SNAP-tag-KDEL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGM55285

   Plasmid

   #153212

   Purpose

   Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6_(GLuc)_INT[BoBS]

   Plasmid

   #68431

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTU2-A-RFP (p15A origin)

   Plasmid

   #74090

   Purpose

   pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   R0010 Const. Promoter controlling RFP

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE476

   Plasmid

   #153267

   Purpose

   M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid

    #139787

    Purpose

    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Jeannie Lee, Arneet Saltzman

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    April 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IBU-SNAP-Rab7

    Plasmid

    #228141

    Purpose

    Expresses SNAP-Rab7 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Rab7 (Rab7 Mouse)
    

    Use

    Retroviral

    Tags

    SNAP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TRE-mNeonGreen-WPRE

    Plasmid

    #193341

    Purpose

    AAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGM65905

    Plasmid

    #153217

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. STAGR_SAMScaffold_hU6

    Plasmid

    #102843

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_hU6promoter
    

    Use

    As pcr template

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVG122

    Plasmid

    #211765

    Purpose

    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    strongLOV
    

    Use

    For single-copy integration (if cut with pmei)

    Tags

    SV40 NLS and VP16

    Expression

    Yeast

    Mutation

    compared to EL222, strongLOV contains Glu84Asp mu…

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO mouse shRNA 2 raptor

    Plasmid

    #21340

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    raptor shRNA 2 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

    Plasmid

    #113867

    Purpose

    Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    NLuc
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. STAgR_tdTomato

    Plasmid

    #102993

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Promoter

    U6

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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