We narrowed to 7,673 results for: ski

1. pT3EF1aH-myr-Akt

   Plasmid

   #179909

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

   Depositor

   Xin Chen

   Article

   Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

   Insert

   Akt1 (Akt1 Mouse)
   

   Tags

   HA and Myr

   Expression

   Mammalian

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

   Plasmid

   #193294

   Purpose

   episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
   

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDMJ2-SARS-CoV-2-Spike (HV.1)

   Plasmid

   #241116

   Purpose

   Expresses SARS-CoV-2 spike (HV.1) protein for creating pseudotyped virus

   Depositor

   Jeremy Luban

   Article

   Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

   Insert

   Spike (HV.1) (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   T19I,L24del,P25del,P26del,A27S,Q52H,V83A,G142D,Y1…

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCM-CLYBL-NGN2-BRN3A

   Plasmid

   #165597

   Purpose

   Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

   Depositor

   Carsten Bönnemann

   Article

   Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   BRN3A (POU4F1 Human)
   EGFP
   Puromycin N-acetyl transferase
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

   Plasmid

   #210017

   Purpose

   episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Expression

   Mammalian

   Mutation

   Sox2A61V is a point mutant of Sox2 that enhances …

   Promoter

   CAG

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-mEGFP-DVL1-WT

   Plasmid

   #194586

   Purpose

   Mammalian Expression of mEGFP-DVL1 WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   DVL1 (DVL1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-mEGFP-HMGB1-WT-IDR

   Plasmid

   #194549

   Purpose

   Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   HMGB1 (HMGB1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

   Plasmid

   #112022

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha/beta into TRBC1 HDRDT
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

   Plasmid

   #210016

   Purpose

   episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. NYESO alpha into TRAC HDRT Source (pTR 223)

    Plasmid

    #112023

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NYESO beta into TRBC1 HDRT Source (pTR 277)

    Plasmid

    #112024

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta into TRBC1 HDRT
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-45b-mEGFP-HMGB1-WT

    Plasmid

    #194543

    Purpose

    Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-mEGFP-HMGB1-WT-Full-length

    Plasmid

    #194548

    Purpose

    Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-mEGFP-RUNX1-WT

    Plasmid

    #194574

    Purpose

    Mammalian Expression of mEGFP-RUNX1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    RUNX1 (RUNX1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-mEGFP-SOX2-WT

    Plasmid

    #194580

    Purpose

    Mammalian Expression of mEGFP- SOX2 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    SOX2 (SOX2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pαH-rS2d-HexaPro

    Plasmid

    #183515

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3-SARS2-B.1.617.1

    Plasmid

    #172319

    Purpose

    expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.1 strain (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

    Promoter

    CMV

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_HSD17B10_WT_V5

    Plasmid

    #82941

    Purpose

    Gateway Donor vector containing HSD17B10, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    HSD17B10 (HSD17B10 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-mEGFP-CALR-WT

    Plasmid

    #194578

    Purpose

    Mammalian Expression of mEGFP-CALR WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    CALR (CALR Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-mEGFP-HMGB3-WT

    Plasmid

    #194564

    Purpose

    Mammalian Expression of mEGFP-HMGB3 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB3 (HMGB3 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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