We narrowed to 10,183 results for: yeast

1. pHflu1

   Plasmid

   #203878

   Purpose

   pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer

   Depositor

   Bogumil Karas

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. KBB258

   Plasmid

   #185072

   Purpose

   Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion

   Depositor

   Ken Belanger

   Insert

   NUP1
   

   Tags

   GST

   Expression

   Yeast

   Mutation

   Nup1 N-term+FXFG repeats

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HCP2

   Plasmid

   #166104

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCP3

   Plasmid

   #166105

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HCP7

   Plasmid

   #166109

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

   Depositor

   Matthias Heinemann

   Insert

   Whi5-sg106 (WHI5 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. brr2-C520R pRS313

   Plasmid

   #111412

   Purpose

   brr2-C520R (endogenous promoter)

   Depositor

   Christine Guthrie

   Article

   Mayerle et al bioRxiv 2018. 354514

   Insert

   BRR2
   

   Expression

   Yeast

   Mutation

   C520R aa mutation

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. brr2-N1104A pRS313

   Plasmid

   #111414

   Purpose

   brr2-N1104A (endogenous promoter)

   Depositor

   Christine Guthrie

   Article

   Mayerle et al bioRxiv 2018. 354514

   Insert

   BRR2
   

   Expression

   Yeast

   Mutation

   N1104A aa mutation

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. brr2-R1107L pRS313

   Plasmid

   #111416

   Purpose

   brr2-R1107L (endogenous promoter)

   Depositor

   Christine Guthrie

   Article

   Mayerle et al bioRxiv 2018. 354514

   Insert

   BRR2
   

   Expression

   Yeast

   Mutation

   R1107L aa mutation

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREP81xV5_ccdB

   Plasmid

   #79019

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pREP41xV5_ccdB

    Plasmid

    #79017

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREP82xV5_ccdB

    Plasmid

    #79020

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREP42xV5_ccdB

    Plasmid

    #79018

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK275

    Plasmid

    #73363

    Purpose

    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)

    Depositor

    T. J. Kappock

    Insert

    delta-8 sphingolipid desaturase
    

    Expression

    Yeast

    Mutation

    missing residues 1-6

    Promoter

    GAL1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. brr2-Q904E pRS313

    Plasmid

    #111413

    Purpose

    brr2-Q904E (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    Q904E aa mutation

    Availability

    Academic Institutions and Nonprofits only

    

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15. brr2-R1107A pRS313

    Plasmid

    #111415

    Purpose

    brr2-R1107A (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    R1107A aa mutation

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGR595

    Plasmid

    #213785

    Purpose

    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid

    #181707

    Purpose

    Galactose inducible expression of Kar2-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Kar2-Beta-Amyloid
    

    Expression

    Yeast

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. YIp204-PADH1-AFB2

    Plasmid

    #99531

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS303-PADH1-AFB2

    Plasmid

    #99530

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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