We narrowed to 15,775 results for: 110

1. psiCHECK2-E2F3 3'UTR

   Plasmid

   #29470

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F3 3'UTR (E2F3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pTER GAMMA-TUBULIN shRNA Human

   Plasmid

   #87955

   Purpose

   reduced the expression of gamma-tubulin in human cells

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   sh gamma-tubulin (TUBG1 Human)
   

   Use

   Tags

   No-tag

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene.…

   Promoter

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-neo-myc-LC3(deltaC22, G120A)

   Plasmid

   #45449

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Encodes amino acids 1-120, Glycine at position 12…

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMXs-hu-c-Myc-dN2

   Plasmid

   #50774

   Purpose

   retroviral expression of human c-Myc dN2 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN2 (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 42-439

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-hu-c-Myc-dN1

   Plasmid

   #50773

   Purpose

   retroviral expression of human c-Myc dN1 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN1 (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 14-439

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN-EYFP-hPMLIII

   Plasmid

   #103808

   Purpose

   BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-hPMLIII (CIB1, PML Mustard Weed, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1068 pGL3 FasL promoter (no SV40 prom)

   Plasmid

   #9029

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   Fas ligand promoter (FASLG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Removed the SV40 promoter from pGL3

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-6P-1-INTS3-C

   Plasmid

   #128417

   Purpose

   Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 (INTS3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   INTS3 C-terminal region (514-1042 aa)

   Promoter

   Tac promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST27-GST-ATXN1 FL(92Q)-S776D

   Plasmid

   #21757

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 92 Q(Glutamines) and…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

10. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

    Plasmid

    #166801

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    S730A

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE GSG1l-GFP KI

    Plasmid

    #131492

    Purpose

    Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gsg1l Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pN1 VAMP2 R125TAG

    Plasmid

    #69877

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Amber and Ochre stop codons in the former linker …

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

14. LIC-Z-YFP

    Plasmid

    #153544

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    Venus YFP

    Expression

    Mammalian

    Mutation

    with mCherry in LIC-Z replaced by YFP

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST27-GST-ATXN1 FL(30Q)-S776D

    Plasmid

    #21755

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

16. PIK3CD gRNA (BRDN0001146545)

    Plasmid

    #76165

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3CD

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT-TODestFLAGHA_RBPMS

    Plasmid

    #59388

    Purpose

    Destination vector with RBPMS for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMMP-NGFR:LMP1-IRES-mRFP

    Plasmid

    #36974

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Insert

    NGFR:LMP1 (NGFR, LMP-1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NGFR:LMP1 chimeric receptor consists of aa 1–276 …

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIBN-TetR-tagRFP-T

    Plasmid

    #103809

    Purpose

    'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-TetR-tagRFP-T (CIB1, tetR E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIBN-EYFP-TetR

    Plasmid

    #103798

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-TetR (CIB1, tetR E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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