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Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorInsertmCherry-GFP-LC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-FCRL2-Fc(DAPA)-AviTag-6xHis
Plasmid#156812PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertFCRL2 (FCRL2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2iApowriter2(#336)
Plasmid#184104Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesisTagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Apowriter2(#340)
Plasmid#184105Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWtEos3N(#486)
Plasmid#184107Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWChe(#488)
Plasmid#184108Purposecyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C2_8
Plasmid#60286PurposeThis plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.DepositorInsertRAP1A inactive enhancer (RAP1A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR D-TOPO-C2_8
Plasmid#60249PurposeThis plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.DepositorInsertRAP1A inactive enhancer (RAP1A Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCX459
Plasmid#229214PurposeCRISPR array plasmid for MAP1B (Spacers 1-24)DepositorInsertCRISPR array plasmid for MAP1B (Spacers 1-24)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCX460
Plasmid#229215PurposeCRISPR array plasmid for MAP1B (Spacers 25-48)DepositorInsertCRISPR array plasmid for MAP1B (Spacers 25-48)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215014PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-cIAP2mut
Plasmid#8336DepositorInsertciap2mut (BIRC3 Human)
UseTagsGSTExpressionBacterialMutationH574APromoterAvailable sinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1 hciap2mut
Plasmid#8339DepositorInsertciap2mut (BIRC3 Human)
UseTagsmycExpressionMammalianMutationH574APromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3D10
Plasmid#71004PurposeGateway entry cloneDepositorInsertMED23 (MED23 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-SRGAP3-EGFP
Plasmid#168504PurposeExpression of full length SRGAP3 with a C-terminal EGFP fusionDepositorInsertSRGAP3 (SRGAP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP3FBAR-EGFP
Plasmid#168506PurposeExpression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusionDepositorInsertSRGAP3-FBAR (SRGAP3 Human)
UseTagsEGFPExpressionMammalianMutationaa 1-492PromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-shortSUMO1
Plasmid#228951Purposeexpress a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimizedDepositorInsertSUMO1 (SUMO1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationdeleted amino acids 1-17PromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only