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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 681 - 700 of 2493 results

  2. pKm-HCBE

    Plasmid
    #170333
    Purpose
    a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti
    Depositor
    Deqiang Duanmu
    Publication
    Wang et al Front Microbiol. 2021 Jun 18;12:686008.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rhizobium codon-optimized CBEs
    Use
    CRISPR
    Mutation
    Cas9n-D10A
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170333-plasmid-map-sequence-id-341069

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  4. CABE-T3.1

    Plasmid
    #193267
    Purpose
    Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter
    Depositor
    Nicole Gaudelli
    Publication
    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CABE-T3.1
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193267-plasmid-map-sequence-id-379830

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  5. pCDNA3-hTLR4-VenusFlag

    Plasmid
    #197640
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TLR4 (TLR4 Human)
    Tags
    Venus-Flag
    Expression
    Mammalian
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197640-plasmid-map-sequence-id-391702

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  7. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  8. miniABEmax (pRZ900)

    Plasmid
    #131311
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131311-plasmid-map-sequence-id-254652

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  9. HSD17B10

    Plasmid
    #39153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B10 (HSD17B10 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pYPQ266E

    Plasmid
    #161521
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing
    Depositor
    Yiping Qi
    Publication
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161521-plasmid-map-sequence-id-325638

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  12. pKm-HGBE

    Plasmid
    #170334
    Purpose
    a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti
    Depositor
    Deqiang Duanmu
    Publication
    Wang et al Front Microbiol. 2021 Jun 18;12:686008.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rhizobium codon-optimized GBEs
    Use
    CRISPR
    Mutation
    Cas9n-D10A
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    170334-plasmid-map-sequence-id-339559

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  13. pCR1054

    Plasmid
    #71822
    Purpose
    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71822-plasmid-map-sequence-id-131019

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  14. pCMV-Target-AIDmax

    Plasmid
    #139107
    Purpose
    Mammalian Target-AIDmax expression vector (pSI-626)
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    139107-plasmid-map-sequence-id-296574

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  15. pCMV-BE3-FNLS(RA)

    Plasmid
    #112671
    Purpose
    CMV expression vector for BE3-FNLS construct (codon optimized)
    Depositor
    Lukas Dow
    Publication
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-FNLS
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    NLS sequence at the N-terminus and D10A
    Promoter
    CMV
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112671-plasmid-map-sequence-id-217194

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  17. pMOD_A0601

    Plasmid
    #91016
    Purpose
    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-P2A-AtCas9_D10A nickase
    Use
    CRISPR
    Mutation
    D10A
    Promoter
    35S
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91016-plasmid-map-sequence-id-181770

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  18. pCMV-hA3A-BE3-Y130F

    Plasmid
    #113428
    Purpose
    Expresses hA3A-BE3-Y130F in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A-BE3-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    hAPOBEC3A_Y130F
    Promoter
    CMV
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113428-plasmid-map-sequence-id-217473

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  19. pKm-HABE

    Plasmid
    #170332
    Purpose
    a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti
    Depositor
    Deqiang Duanmu
    Publication
    Wang et al Front Microbiol. 2021 Jun 18;12:686008.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rhizobium codon-optimized ABEs
    Use
    CRISPR
    Mutation
    Cas9n-D10A
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170332-plasmid-map-sequence-id-341070

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  20. pH-ABE8e

    Plasmid
    #200774
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Publication
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200774-plasmid-map-sequence-id-399597

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Showing: 681 - 700 of 2493 results