We narrowed to 874 results for: VILL

1. pEGFP-C1-G2BRgp78

   Plasmid

   #185344

   Purpose

   Expression of soluble G2BR, amino acids 575-600 of human gp78

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   gp78 aa 574-600 (G2BR) (AMFR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 575-600 only

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDUET-GST-AUP1 379-410:Ube2G2

   Plasmid

   #185335

   Purpose

   Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-GG-hUbC-EBPF2-gRNA-RBMS3

   Plasmid

   #185557

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   RBMS3 gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-GG-hUbC-EBPF2-gRNA-HHIP

   Plasmid

   #185551

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting HHIP

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   HHIP gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-GG-hUbC-EBPF2-gRNA-ADGRG6

   Plasmid

   #185554

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   ADGRG6 gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

   Plasmid

   #176150

   Purpose

   N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Mutation in the PAM site used by POLB gRNA1

   Promoter

   EF1A

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

   Plasmid

   #176087

   Purpose

   EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in Lys72 to Ala, and mutation in the PAM…

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Hygro-EF1A-XL1/BRCT1-Linker-eGFP

   Plasmid

   #176084

   Purpose

   EGFP fused to the C-terminus of a XL1/BRCT1 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   XL1/BRCT1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-Mm_MyoGEF(T575A)

   Plasmid

   #136328

   Purpose

   Mouse MyoGEF-T575A mutant in Gateway pENTR

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   PLEKHG6 (Plekhg6 Mouse)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   T575A

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDY0663 ACTB atgRNA with v2 scaffold

    Plasmid

    #179108

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti.DTR.GFP

    Plasmid

    #201962

    Purpose

    Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

    Depositor

    Bernhard Schmierer

    Article

    Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

    Insert

    Diptheria Toxin Receptor
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Bsd-CMV-MDM4

    Plasmid

    #202669

    Purpose

    MDM4 overexpression vector with blasticidin resistance

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 regulator of p53 (MDM4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDY0216 PASTEv1 PE2-P2A-Bxb1

    Plasmid

    #179104

    Purpose

    PASTEv1 PE2-P2A-Bxb1

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-PE2-P2A-Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-TetOne-Puro-MDM4

    Plasmid

    #195140

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pDY0207 ACTB atgRNA with v1 scaffold

    Plasmid

    #179107

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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