We narrowed to 15,697 results for: NOL

1. pMV_AA088

   Plasmid

   #216104

   Purpose

   Knockout, EF1a-driven Cas9 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIEG3-c-Jun

   Plasmid

   #40348

   Depositor

   Alexander Dent

   Article

   Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

   Insert

   c-Jun (JUN Human)
   

   Use

   Retroviral

   Tags

   IRES eGFP

   Expression

   Mammalian

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJA167

   Plasmid

   #128536

   Purpose

   Expresses DsRed

   Depositor

   Marcos Alcocer

   Article

   Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

   Insert

   NFAT-DsRed reporter
   

   Expression

   Mammalian

   Promoter

   SV40-NFAT

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. DR274

   Plasmid

   #42250

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR; Zebrafish expression

   Expression

   Worm

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB512

   Plasmid

   #74854

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSTARR-seq_SCP1 vector_blocking 4

   Plasmid

   #99319

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Expression

   Mammalian

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Expression

   Yeast

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF-Bos TRIF Flag

    Plasmid

    #41550

    Depositor

    Kate Fitzgerald, Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    Trif (TICAM1 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92392

    Purpose

    produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Human Synapsin

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SaKKH-ABE8e

    Plasmid

    #138502

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSaCas9 (KKH)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK198 (GAL-OsTIR1)

    Plasmid

    #140655

    Purpose

    GAL-OsTIR1(WT)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT)
    

    Expression

    Yeast

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCH61 (multiAsCas12a-KRAB piggyBac)

    Plasmid

    #217332

    Purpose

    piggyBac expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #179686

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    T1413I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB402

    Plasmid

    #74796

    Purpose

    Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-Tight-Puro_MYRF

    Plasmid

    #64843

    Purpose

    A lentiviral vector for the expression of mouse MYRF under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    MYRF (Myrf Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

    Plasmid

    #191159

    Purpose

    Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:EGFP.s2:mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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