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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMS10

Plasmid

#230074

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mNeonGreen fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG and mNeonGreen

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMS02

Plasmid

#230066

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-PacI

Plasmid

#170100

Purpose

deGFP reporter plasmid with unique PacI restriction enzyme recognition site

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas5

Plasmid

#170090

Purpose

expresses E. coli type I-E Cas5

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas5

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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ELP-TEMP/pcDNA3.1(-)

Plasmid

#178434

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

Insert

ELP-TEMP

Tags

No tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBM-strep-UCP1

Plasmid

#216207

Purpose

for expression of human strep-tagged UCP1 protein in BacMam system

Depositor

Lei Chen

Article

Kang et al Nature. 2023 Aug;620(7972):226-231. doi:

Insert

UCP1 (UCP1 Human)

Tags

strep

Expression

Mammalian

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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JGI_018319

Plasmid

#158837

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Mex_1740

Use

Synthetic Biology

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSEED-ALFA:HA

Plasmid

#219646

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

ALFA:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-mGreenLantern:ALFA

Plasmid

#219644

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern:ALFA SEED Cassette

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP:ALFA

Plasmid

#219642

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPlantPOP_STARBURST

Plasmid

#241284

Purpose

UBQ10 driven expression of STARBURST, a poly-cistronic fungal bioluminescense pathway, in plants

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Insert

STARBURST

Expression

Plant

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pVP141

Plasmid

#222079

Purpose

Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

Insert

dCas9-PmCDA1, sfGFP and sacB

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA Scramble

Plasmid

#162011

Purpose

Lentiviral negative control vector containing scramble shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

scramble shRNA

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO AMPKa (11-281)

Plasmid

#177851

Purpose

Bacterial Expression of AMPKa1

Depositor

Karsten Melcher

Article

Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

Insert

AMPK a1 (PRKAA1 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSmartBAC-S

Plasmid

#107268

Purpose

Broad host range bacterial artificial chromosome vector

Depositor

Ron Godiska, Mark Liles

Article

Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

Type

Empty backbone

Expression

Bacterial

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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