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pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-xlH2B(1-114)_K114A

Plasmid

#118217

Purpose

Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

xlH2B(1-114)

Tags

Mxe-GyrA intein fused to chitin binding domain

Expression

Bacterial

Mutation

Lysine 114 to alanine

Promoter

T7

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBlue-hFX(AA88)-HA

Plasmid

#15402

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pLV_UCOE-EF1a_3XHA-FPR1

Plasmid

#254851

Purpose

Mammalian expression of the human formyl peptide receptor FPR1 with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Akdogan et al Commun Biol. 2026 Mar 25. doi: 10.1038/s

Insert

3xHA-FPR1 (FPR1 Human)

Use

Lentiviral

Tags

3x HA tag

Expression

Mammalian

Promoter

EF1 alpha

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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LIG4 HAL-T2A-GFP-SV40pA-HAR

Plasmid

#225357

Purpose

This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the SelectRepair knockout GFP approach.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

T2A-GFP-SV40pA

Use

CRISPR

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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LIG4 HAL-T2A-HYG-SV40pA-HAR

Plasmid

#225358

Purpose

This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the hygromycin B SelectRepair knockout approach.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

T2A-HYG-SV40pA

Use

CRISPR

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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LIG4 HAL-T2A-NEO-SV40pA-HAR

Plasmid

#225359

Purpose

This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the neomycin SelectRepair knockout approach.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

T2A-NEO-SV40pA

Use

CRISPR

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pX459_LIG4_Exon3

Plasmid

#225363

Purpose

pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

LIG4 (LIG4 Human)

Use

CRISPR

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE FLAG HA p100 (Ser707/711Ala)

Plasmid

#236469

Purpose

stable expression of p100 using virus infection

Depositor

Michele Pagano

Article

Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

Insert

p100 (NFKB2 Human)

Expression

Mammalian

Mutation

Ser707/711Ala

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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