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B5-5

Plasmid

#111484

Purpose

translational initiation factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 3

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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B3-1

Plasmid

#111471

Purpose

translational initiation factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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B4-5

Plasmid

#111479

Purpose

ribosome recycling factor

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

ribosome recycling factor

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-11

Plasmid

#79926

Purpose

Palm1-Late1-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pEB1-mClover3

Plasmid

#103994

Purpose

Plasmid encoding mClover3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mClover3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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RTA

Plasmid

#64029

Purpose

Expression of Ricin A Chain (RTA) in E. coli

Depositor

Vern Schramm

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Dec 1;106

Insert

Ricin A Chain

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
prNFL-EGFP

Plasmid

#132605

Purpose

Expresses EGFP-tagged rat NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSA101

Plasmid

#59787

Purpose

drives ZIF-1 protein in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
zif-1 coding (zif-1 Nematode)
unc-54 3' UTR

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTetR193-Wasabi

Plasmid

#86105

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR I193N

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

I193N

Promoter

ptetA and ptetR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-AVT1 chr I

Plasmid

#41898

Purpose

Yeast expression of AVT1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

AVT1 (AVT1 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLacI241-Wasabi

Plasmid

#86102

Purpose

mWasabi reporter gene gated by temperature-sensitive LacI A241T

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

LacIts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A241T

Promoter

T7A1O4/O3 and pLacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMVV098

Plasmid

#215304

Purpose

Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PPhIF

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO-hCALML3

Plasmid

#47601

Depositor

Emanuel Strehler

Article

Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

Insert

CALM3 (CALML3 Human)

Expression

Mammalian

Promoter

tet-inducible CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pAGT5439

Plasmid

#192351

Purpose

L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-tOCS_CGCT

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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B1-1

Plasmid

#111463

Purpose

Val-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Val-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-MNN9-msGFP2

Plasmid

#177105

Purpose

Expresses Mnn9-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

MNN9 (MNN9 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Tags

Myc

Expression

Bacterial

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only
pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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B1-2

Plasmid

#111464

Purpose

Met-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
UIMLx2

Plasmid

#174888

Purpose

UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

Depositor

Peter Kaiser

Article

Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

Insert

Met4 UIML

Tags

Avi-6xHis-Smt3

Expression

Bacterial

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-LaminB1

Plasmid

#184460

Purpose

Nuclear membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-sfGFP

Plasmid

#103983

Purpose

Plasmid encoding sfGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

sfGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-248

Plasmid

#104709

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 C13A (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

C13A, BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-21

Plasmid

#104707

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 WT (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-UBC1

Plasmid

#104666

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC1 (UBC1 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
B7-4

Plasmid

#111492

Purpose

Met-tRNA formyltransferase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA formyltransferase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-EPN-51

Plasmid

#79966

Purpose

Late1-O3-33-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-EPN-09

Plasmid

#79924

Purpose

Palm3-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-09

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pAG306-GPD-VPH2 chr I

Plasmid

#41897

Purpose

Yeast expression of VPH2.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VPH2 (VPH2 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge