We narrowed to 14,379 results for: Cre

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M13cp-dg2 hp

Plasmid

#218094

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pII

Depositor

Kevin Esvelt

Publication

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDHJS4 AS+

Plasmid

#78242

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Publication

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS-

Plasmid

#78245

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Publication

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS+

Plasmid

#78244

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Publication

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AS-

Plasmid

#78243

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Publication

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available since

June 6, 2016

Availability

Academic Institutions and Nonprofits only
pMA1662

Plasmid

#46878

Purpose

Retroviral vector, Hygromycin resistance

Depositor

Mikhail Alexeyev, Troy Stevens

Publication

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Type

Empty backbone

Use

Retroviral

Available since

Sept. 30, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pGL2314

Plasmid

#137054

Purpose

loxP (empty) expression cassette: c-terminal 6xHA

Depositor

Jeremy Mottram

Publication

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Type

Empty backbone

Use

Leishmania floxed gene expression

Tags

6xHA

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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p029 End-1::EnR

Plasmid

#11029

Depositor

Ramesh Shivdasani

Publication

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

End1::EnR (end-1, en Fly, Nematode)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Dominant inhibitory GATA-binding protein End1::En…

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pAC025

Plasmid

#227597

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

Depositor

Brittany Adamson

Publication

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLib6.6

Plasmid

#176652

Purpose

Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Publication

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG

Plasmid

#175501

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIVEX-anchor

Plasmid

#46844

Purpose

Template for the amplification of spiking anchors in BeSD

Depositor

Florian Hollfelder

Publication

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Type

Empty backbone

Use

In vitro expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pRDA_449

Plasmid

#179093

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Publication

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpG

Use

CRISPR and Lentiviral

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Consensus

Plasmid

#176678

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Publication

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-7

Plasmid

#163135

Purpose

pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Nanobody, C-IgAP autotransporter

Expression

Bacterial

Promoter

P(rhaB)

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX_313-EGFP

Plasmid

#158580

Purpose

Lentiviral expression of EGFP

Depositor

John Doench, David Root

Publication

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pV2A-T

Plasmid

#153022

Purpose

Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

Depositor

Vito Valiante

Publication

Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGMC00029 (aka pRC0610)

Plasmid

#200834

Purpose

Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

Depositor

Raj Chari

Publication

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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