We narrowed to 36,268 results for: Tec

1. pCellFree_G03 CSNK2A1

   Plasmid

   #67085

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CSNK2A1 (CSNK2A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET28b+R3(R112C/T180C)

   Plasmid

   #108195

   Purpose

   Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA3(R112C/T180C) fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAL2209

   Plasmid

   #36672

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HOXA13-TALEN_Right (HOXA13 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pTrc99S-ssDsbA-PD-4xDQNAT

   Plasmid

   #128399

   Purpose

   Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   H. influenzae protein D
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrc99S-ssDsbA-PorA-4xDQNAT

   Plasmid

   #128400

   Purpose

   Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   N. meningitidis PorA
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

   Plasmid

   #131618

   Purpose

   Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR[dsxM]
   NeoR[traF]
   

   Expression

   Insect

   Promoter

   Opie2

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6Rosa-CAG-Gal4-Rad18UBD

   Plasmid

   #119012

   Purpose

   Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TAL2323

   Plasmid

   #36749

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FOXO1-TALEN_Right (FOXO1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. TAL2326

   Plasmid

   #36752

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MET-TALEN_Left (Met Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   July 11, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TAL2327

    Plasmid

    #36753

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MET-TALEN_Right (Met Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLPC myc-mRAP1 I312R

    Plasmid

    #73137

    Purpose

    Expression of myc-tagged mRAP1 with single point mutatoin I312R

    Depositor

    Agnel Sfeir

    Article

    Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

    Insert

    Rap1 (Terf2ip Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    isoleucine changed to an arginine at amino acid 3…

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

12. TAL2271

    Plasmid

    #36708

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    GLI1-TALEN_Right (GLI1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. fgfprom luc

    Plasmid

    #69446

    Purpose

    basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Insert

    minimal fgf4 promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL2200

    Plasmid

    #36663

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    AXIN2-TALEN_Left (AXIN2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TAL2201

    Plasmid

    #36664

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    AXIN2-TALEN_Right (AXIN2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pRSET FLIPglu-600uDelta13 Aphrodite

    Plasmid

    #13570

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13 Aphrodite
    

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVsc.U6-sgGTTG.CMV/CB-Gluc

    Plasmid

    #121512

    Purpose

    Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgGTTG
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bambi NGFR

    Plasmid

    #44628

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    Bambi (Bambi Mouse)
    

    Use

    Retroviral

    Tags

    IRES and truncated NGFR

    Expression

    Mammalian

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Gal4pBS34-Pleum

    Plasmid

    #60334

    Purpose

    Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCellFree_G03 CTNNB1

    Plasmid

    #67119

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only