166,461 results

1. pD649-HAsp-IL1R1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156756

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   IL1R1 (IL1R1 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX419

   Plasmid

   #229216

   Purpose

   All-in-one plasmid for labeling of XIST RNA via Csm complex

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_mCherry-P300

   Plasmid

   #238258

   Purpose

   For overexpression of mCherry-P300

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry-P300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRY2-GFP-homer1c

   Plasmid

   #89442

   Purpose

   Localizes CRY2 to excitatory synapses

   Depositor

   Matthew Kennedy

   Article

   Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

   Insert

   CRY2-GFP-homer1c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains Full length CRY2

   Promoter

   CAG

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYES2-Ub-M-GFP

   Plasmid

   #11952

   Depositor

   Nico Dantuma

   Article

   Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

   Insert

   Ub-M-GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Ubiquitin fused to N-terminus of GFP. Methionine …

   Promoter

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P-1 SARS-CoV 3CLpro

   Plasmid

   #61692

   Purpose

   inducible expression of SARS 3CLpro protease

   Depositor

   Michael Johnson

   Article

   Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

   Insert

   SARS 3CLpro protease
   

   Use

   Tags

   GST and HIS

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2364

   Plasmid

   #142051

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB1 (CREB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. YIplac211-yoHaloTag-TLG1

    Plasmid

    #105277

    Purpose

    Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    yoHaloTag

    Expression

    Yeast

    Mutation

    HaloTag has yeast optimized codons

    Promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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