We narrowed to 29,714 results for: REP

1. pdCas9-M-3H5

   Plasmid

   #73429

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 3H5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-M-1D4

   Plasmid

   #73433

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1D4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pdCas9-M-1E4

   Plasmid

   #73436

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1E4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pdCas9-M-5F5

   Plasmid

   #73432

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 5F5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGM190oriT

   Plasmid

   #115679

   Purpose

   E. coli-Streptomyces shuttle vector for gene expression

   Depositor

   Günther Muth

   Insert

   oriT, aphII, tsr
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tipA

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGMGUS

   Plasmid

   #115678

   Purpose

   E.coli-Streptomyces shuttle vector, for gene knock out experiments

   Depositor

   Günther Muth

   Insert

   gusA, aac(3)IV, tsr
   

   Use

   Gene knock out

   Tags

   Expression

   Mutation

   Promoter

   ermE*

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV uN2CpolyGly op GFP (NIID)

   Plasmid

   #224356

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C with a GGC repeat expansion (100x)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intron (CAG)

   Available since

   Feb. 5, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. PB-TAC-OSKM

   Plasmid

   #80481

   Purpose

   piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-TAC-MKOS

   Plasmid

   #80484

   Purpose

   piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK684

    Plasmid

    #71638

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to valine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK685

    Plasmid

    #71639

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn mutant (TdPurE2-S38N)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to asparagine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK687

    Plasmid

    #71640

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-41 to methionine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK688

    Plasmid

    #71641

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg mutant (TdPurE2-K41R)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-41 to arginine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK695

    Plasmid

    #71644

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-17 to tryptophan

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK696

    Plasmid

    #71645

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes methionine-15 to tryptophan

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK607

    Plasmid

    #71626

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys mutant (TdPurE2-S38C)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to cysteine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK617

    Plasmid

    #71627

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala mutant (TdPurE2-S38A)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to alanine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK643

    Plasmid

    #71628

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr mutant (TdPurE2-S38T)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to threonine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK644

    Plasmid

    #71629

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to aspartate

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC8-SZ

    Plasmid

    #17330

    Depositor

    Takeshi Sano

    Article

    Argarana et al Nucleic Acids Res. 1986 Feb 25. 14(4):18

    Insert

    streptavidin
    

    Use

    For maintenance

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only