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pENTR221-TurquoiseGL

Plasmid

#252336

Purpose

Gateway shuttle vector with mTurquoiseGL.

Depositor

James Conway

Article

Tran et al Small Methods. 2026 Jul 12:e70828. doi:

Insert

mTurquoiseGL

Use

Gateway shuttle vector

Promoter

None

Available Since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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YIplac211-SGM1(-Ypt6_BD)-FRBx2-HA

Plasmid

#255060

Purpose

Remove the Sgm1 Ypt6 binding domain sequence and insert a C-terminal FRBx2-HA tag at SGM1 by popin/popout.

Depositor

Benjamin Glick

Insert

Sgm1(-Ypt6_BD)-FRBx2-HA

Expression

Yeast

Mutation

Sgm1 Ypt6 binding domain removed

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_holD-GS8-holC_holB

Plasmid

#254008

Purpose

T7 co-expression vector encoding ψ-GS8-χ fusion (MCS1) and holB (MCS2) for clamp loader assembly studies.

Depositor

Linda Bloom

Article

Padgett-Pagliai et al Nucleic Acids Res. 2026 May 20;54(10):gk

Insert

holD-GS8-holC + holB

Expression

Bacterial

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_holD-GS12-holC_holB

Plasmid

#254009

Purpose

T7 co-expression vector encoding ψ-GS12-χ fusion (MCS1) and holB (MCS2) for clamp loader reconstitution.

Depositor

Linda Bloom

Article

Padgett-Pagliai et al Nucleic Acids Res. 2026 May 20;54(10):gk

Insert

holD-GS12-holC + holB

Expression

Bacterial

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-HA TUB1

Plasmid

#246173

Purpose

Used for transfection of cells for expression N-HA TUB1

Depositor

Sheng-cai Lin

Article

Qu et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

TUB1

Tags

HA

Expression

Mammalian

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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MBP-GFP-RGG

Plasmid

#242453

Purpose

Expresses the MBP-GFP-RGG fusion protein.

Depositor

Benjamin Schuster

Article

Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

Insert

MBP-GFP-RGG

Tags

His and MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GFP-RGG

Plasmid

#242454

Purpose

Expresses the GST-GFP-RGG fusion protein.

Depositor

Benjamin Schuster

Article

Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

Insert

GST-GFP-RGG

Tags

GST and His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-DDX6-sgDDX6-1 mut

Plasmid

#240009

Purpose

Expresses human DDX6 fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-sgDDX6-1 mut

Use

Lentiviral

Tags

3X Flag

Mutation

TTAAAAAAAGATAACATC

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLY071c

Plasmid

#227940

Purpose

SICLOPPS precursor for cyclo-CHVVGVI

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CHVVGVI

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLY071e

Plasmid

#227941

Purpose

SICLOPPS precursor for cyclo-CDLGVFR

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CDLGVFR

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLY090a

Plasmid

#227938

Purpose

SICLOPPS precursor for cyclo-CRVWCAR

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CRVWCAR

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2371

Plasmid

#225637

Purpose

High Copy number plasmid containing Ple388 (PITX3 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple388 (PITX3 Human)

Use

Unspecified

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K-TDH3pr-mNeon

Plasmid

#194534

Purpose

Low copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42K-TDH3pr-mNeon

Plasmid

#194535

Purpose

High copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41N-TDH3pr-mNeon

Plasmid

#194536

Purpose

Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42N-TDH3pr-mNeon

Plasmid

#194537

Purpose

High copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41H-TDH3pr-mNeon

Plasmid

#194538

Purpose

Low copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGM258

Plasmid

#220176

Purpose

Expresses exogenous C-terminal mutant TTC1 (I271D)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

I271D

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGM133

Plasmid

#220173

Purpose

CRISRPi guide RNA against TTC1

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (sgRNA) (TTC1 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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