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pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

Plasmid

#111392

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
ChETA-EYFP

Use

AAV

Tags

EYFP and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75123

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

3x FLAG-tag

Mutation

bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

Plasmid

#75126

Purpose

Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Mutation

SET domain replaced with the SET domain of EZH1

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
119_pAAV-ProC25-CatCh-GFP-WPRE

Plasmid

#125960

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC25

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1503

Plasmid

#29299

Purpose

Homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple32 (CLDN5 MiniPromoter) driving a lacZ reporter.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple32 (CLDN5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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62_pAAV-ProA19-CatCh-GFP-WPRE

Plasmid

#125902

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA19

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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60_pAAV-ProA17-CatCh-GFP-WPRE

Plasmid

#125900

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA17

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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3_pAAV-ProA8-CatCh-GFP-WPRE

Plasmid

#125892

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA8

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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IX_pAAV-ProB3-CatCh-GFP-WPRE

Plasmid

#125923

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Promoter

ProB3

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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114_pAAV-ProC22-CatCh-GFP-WPRE

Plasmid

#125957

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC22

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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35_pAAV-ProC8-CatCh-GFP-WPRE

Plasmid

#125943

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC8

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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140_pAAV-ProA33-CatCh-GFP-WPRE

Plasmid

#125916

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA33

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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167_pAAV-ProC34-CatCh-GFP-WPRE

Plasmid

#125968

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC34

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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13_pAAV-ProC71-CatCh-GFP-WPRE

Plasmid

#125976

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC71

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Cer(1-158)-beta-5 in pcDNAI/Amp

Plasmid

#55708

Purpose

An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

mCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)

Tags

Cer(1-158) was fused to the amino terminus of Gbe…

Expression

Mammalian

Mutation

GBeta-5 was amplified via PCR, which added an N-t…

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 Luciferase

Plasmid

#117072

Purpose

single guide RNA targeting Luciferase

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

Luciferase

Use

CRISPR

Promoter

hU6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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153_pAAV-ProC31-CatCh-GFP-WPRE

Plasmid

#125966

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC31

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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37_pAAV-ProC40-CatCh-GFP-WPRE

Plasmid

#125974

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC40

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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88_pAAV-ProA5-CatCh-GFP-WPRE

Plasmid

#125889

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA5

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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78_pAAV-ProA27-CatCh-GFP-WPRE

Plasmid

#125910

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA27

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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