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Dyn1 K44A

Plasmid

#198340

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Dyn1 D406A

Plasmid

#198341

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Plasmid

#216404

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56, Glutamine 70, 443 and Asp…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (L115R)

Plasmid

#205717

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (WT)

Plasmid

#205716

Purpose

Mammalian expression of XPACK-DNAJC5 (WT)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

XPACK (GCINSKRKD)

Expression

Mammalian

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMX1463

Plasmid

#208805

Purpose

Expresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycin

Depositor

Linda Wordeman

Article

Wordeman et al Mol Biol Cell. 2016 Apr 15;27(8):1300-9.

Insert

FRB (CRP Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_LTB4R(T308A)

Plasmid

#208088

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(T308A) (LTB4R Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T308A

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_LTB4R(S310A)

Plasmid

#208089

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(S310A) (LTB4R Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S310A

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_LTB4R(T308A,S310A)

Plasmid

#208090

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

LTB4R(T308A,S310A) (LTB4R Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T308A, S310A

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R

Plasmid

#208091

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TSAA

Plasmid

#206179

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S192A, S194A

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS

Plasmid

#206180

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S192A

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TASA

Plasmid

#206181

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S194A

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBVboostFGII WPRE 3C-CVB3

Plasmid

#202077

Purpose

Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB3 3C protease with HisTag

Tags

6xHis-tag

Expression

Bacterial, Insect, and Mamm…

Promoter

polh, CAG, T7

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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sgVRK1-hygro

Plasmid

#199640

Purpose

sgRNA guide against VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-hNRG1a

Plasmid

#199235

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

Insert

Neuregulin (NRG1 Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNanos-del50-236_AC

Plasmid

#148459

Purpose

Insect Expression of DmNanos-del50-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-del50-236 (nos Fly)

Expression

Insect

Mutation

sequence contains a deletion of N283 and a mutati…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGTVL2-BIRC2-BIR3

Plasmid

#196367

Purpose

protein expression of the GST-tagged BIR3 domain

Depositor

Stefan Knapp

Article

Schwalm et al Front Cell Dev Biol. 2022 May 26;10:8865

Insert

BIRC2 BIR3 domain

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

contains only S260-Y352

Promoter

T7

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Expression

Worm

Promoter

myo-2

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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deltaVP1d63C + VP2C-GFPdeg

Plasmid

#182481

Purpose

YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-GFPdeg
deltaVP1d63C

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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