We narrowed to 22,830 results for: Sis

1. pFRT-TODestFLAGHA_RBPMS_KDR_K100A

   Plasmid

   #65663

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed lysine 100 to alanine (KDR background)

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT-TODestFLAGHA_RBPMS_KDR_F27A

   Plasmid

   #65661

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed phenylalanine 27 to alanine (KDR backgrou…

   Promoter

   CMV, 2x Tet

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGMCK-0X750-luxAB-DAS+4

   Plasmid

   #49517

   Purpose

   Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   P750-luxAB-DAS+4
   

   Use

   Tags

   DAS+4 (AANDENYSENYADAS)

   Expression

   Bacterial

   Mutation

   Promoter

   P750

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMEH-0X750-luxAB-DAS+4

   Plasmid

   #49519

   Purpose

   Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   P750-luxAB-DAS+4
   

   Use

   Tags

   DAS+4 (AANDENYSENYADAS)

   Expression

   Bacterial

   Mutation

   Promoter

   P750

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFM101

   Plasmid

   #40147

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   gfp::par-2 wild-type RNAi resistant (par-2 Nematode)
   

   Use

   Tags

   GST and eGFP

   Expression

   Bacterial

   Mutation

   Base pairs 6-523 of par-2 were recoded to create …

   Promoter

   tac

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pFM102

   Plasmid

   #40148

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)
   

   Use

   Tags

   GST and eGFP

   Expression

   Bacterial

   Mutation

   R183-5A; base pairs 6-523 of par-2 were recoded t…

   Promoter

   tac

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pOTTC841 - pAAV minP secNLuc

   Plasmid

   #82498

   Purpose

   Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

   Depositor

   Brandon Harvey

   Article

   Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

   Insert

   secNLuc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   c-Fos minimal promoter

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM512

   Plasmid

   #140192

   Purpose

   For curing of Orbit-integrated plasmids and associated drug marker

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   zeocin resistance gene
   Bxb1gp47 (directionality factor)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cloned in gp47 directinality factor in place of R…

   Promoter

   EM7 and Ptet

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1-FLAG

   Plasmid

   #60360

   Purpose

   Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKan-PCUP1-AID*(N)-9myc

    Plasmid

    #99526

    Purpose

    N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Expression

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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