We narrowed to 10,386 results for: Coli
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Plasmid#85087PurposeExpresses PCNA3 protein fused to P450cam (CYP101A1) in E. coliDepositorInsertPCNA3 fused to P450cam
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_GB
Plasmid#78677PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_FB
Plasmid#78676PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_EB
Plasmid#78675PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_AB
Plasmid#78674PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK227
Plasmid#71126PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coliDepositorInsertcitrate synthase
TagsGyrA inteinExpressionBacterialPromoterT7Available SinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_K381A
Plasmid#67732PurposeExpresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged lysine 381 to alaninePromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N300D
Plasmid#67728PurposeExpresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged asparagine 300 to aspartatePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_R373A
Plasmid#67731PurposeExpresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged arginine 373 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N300S
Plasmid#67729PurposeExpresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged asparagine 300 to serinePromoterT7Available SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUNC2001
Plasmid#67280PurposeExpresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcwDepositorInsertVibrio cholera photolyase
Tagsmaltose binding proteinExpressionBacterialMutationnone knownAvailable SinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KQ
Plasmid#64982PurposeExpresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108QAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-OR-MalT-3NT
Plasmid#62309Purposesynthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genomeDepositorInsertsA2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA
UseCRISPRExpressionBacterialPromoterPA2, PPhlF, and pBADAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Guinea Pig 1-88)
Plasmid#62059PurposeExpression of Guinea Pig p53 (residues 1-88) in e. coliDepositorInsertp53
TagsHisExpressionBacterialMutationcontains TAD residues 1-88PromoterT7Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-GFP-Patronin CC 534–868
Plasmid#59054Purposefor E. coli expression of Patronin CC truncationDepositorAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNV-lacZ
Plasmid#53125Purposeretroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertlacZ (lacZ E.coli)
UseRetroviral; Expression in chicken cellsPromoterSNV (spleen necrosis virus)Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1757
Plasmid#53037PurposePconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialPromoterPconNoHindM2 and pConNoHindAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) F234C
Plasmid#170644PurposeExpression of syt1 for purification in E. ColiDepositorAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) V304C
Plasmid#170645PurposeExpression of syt1 for purification in E. ColiDepositorAvailabilityAcademic Institutions and Nonprofits only -
DDR_MKOv4
Pooled Library#140219PurposeKnockout library targeting human DNA damage response and DNA repair genes.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only