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pMXs-9SA.mNgn2-x3HA

Plasmid

#244167

Purpose

Retroviral expression of phospho-mutant mouse Ngn2 with HA tag

Depositor

Kate Galloway

Article

Lende-Dorn et al Cell Rep. 2025 Sep 23;44(9):116226. doi:

Insert

mNgn2 (phospho mutant) (Neurog2 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

S24A, S66A, S192A, S203A, S205A, S215A, S224A, S2…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Scarlet)-ScarlessDsRed

Plasmid

#244938

Purpose

Vector containing a Scarlet-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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KLF17-CRa1-Lsg-MS2

Plasmid

#247476

Purpose

sgRNA1 for KLF17 activation cloned into LsgRNA-MS2 backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA1 targeting KLF17 promoter (KLF17 Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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KLF17-CRa2-Lsg-MS2

Plasmid

#247477

Purpose

gRNA2 for KLF17 activation cloned into LsgRNA-MS2 backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA2 targeting KLF17 promoter (KLF17 Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) WT

Plasmid

#206028

Purpose

Expresses the SNARE motif of wildtype human VAMP3 in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) S44A

Plasmid

#206029

Purpose

Expresses the SNARE motif of human VAMP3 mutant S44A in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Mutation

changes serine 44 to alanine

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) S44E

Plasmid

#206030

Purpose

Expresses the SNARE motif of human VAMP3 mutant S44E in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Mutation

changes serine 44 to glutamic acid

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-ABL2(K281M)

Plasmid

#215468

Purpose

Gateway entry vector with Arg kinase-dead (K281M)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Gateway entry vector

Mutation

K281M (kinase-inactivating)

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR223-ABL2(WT)

Plasmid

#215467

Purpose

Gateway entry vector with Arg wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Gateway entry vector

Mutation

Includes stop codon

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSE152: pFuGW-hUbCp-3CLpro-SV40NLS

Plasmid

#240455

Purpose

SARS-CoV-2 Protease 3CLpro expression plasmid

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Insert

3CLpro

Use

Lentiviral

Promoter

hUbCp

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJV505

Plasmid

#180078

Purpose

MS.SenLT2II_L85Q expression construct to improve transformation in S. enterica LT2

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.SenLT2II_L85Q

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV303

Plasmid

#180079

Purpose

M.SenLT2I expression construct to improve transformation in S. enterica LT2

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.SenLT2I

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV457

Plasmid

#180080

Purpose

M.BbrUI expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUI

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV461

Plasmid

#180081

Purpose

M.BbrUIII expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUIII

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSUPER-Rac1(dog)

Plasmid

#228760

Purpose

A knockdown vector for dog Rac1.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A shRNA targeting the dog Rac1 gene (RAC1 canis lupus)

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12(D199A,C231S)-Clover

Plasmid

#215528

Purpose

Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-Dok1-mScarlet

Plasmid

#215535

Purpose

Expression vector with Dok1 tagged with C-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12(D199A,C231S)

Plasmid

#215518

Purpose

Dox-inducible lentiviral transfer vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12(D199A,C231S)-Clover

Plasmid

#215522

Purpose

Gateway entry vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Tags

Clover

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-Dok1-V2

Plasmid

#215452

Purpose

Expression vector with Dok1 tagged with the BiFC V2 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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