We narrowed to 18,457 results for: multi

1. pMuLE ENTR U6-miR-30 L1-R5

   Plasmid

   #62113

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   RNAi; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) neo with Smad5 CDS

   Plasmid

   #61800

   Purpose

   pcDNA3.1(+) with full length mouse Smad5 CDS

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   Smad5 CDS (Smad5 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pTU2S-b (colE1 origin)

   Plasmid

   #74092

   Purpose

   Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   None

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE671

   Plasmid

   #153237

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-TRE-mTagBFP2-WPRE

   Plasmid

   #193332

   Purpose

   Expresses mTagBFP2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Mutation

   A XhoI site in the coding sequence is silenced

   Promoter

   TRE

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU1/Sm/3' Box_(GLuc)_INT

   Plasmid

   #68438

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U1

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR-Blunt II-TOPO-PTPRD

   Plasmid

   #29705

   Depositor

   Todd Waldman

   Article

   Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

   Insert

   Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
   

   Use

   Cloning vector

   Mutation

   none

   Available Since

   May 26, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pYPQ132D2.0

   Plasmid

   #99890

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEY79

   Plasmid

   #191074

   Purpose

   flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-12 (flp-12 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEY62

    Plasmid

    #191020

    Purpose

    acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-5 (acr-5 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. STAGR_SAMScaffold_hH1

    Plasmid

    #102847

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_hH1
    

    Use

    Pcr template for stagr inserts

    Promoter

    U6

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGM65893

    Plasmid

    #153216

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS5

    Plasmid

    #55750

    Purpose

    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Expression

    Bacterial

    Mutation

    Residues 59-264 of KanR

    Promoter

    T7-lac

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. KTK_136

    Plasmid

    #180520

    Purpose

    Empty backbone for D1.1 cloning, contains sfGFP dropout

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid

    #68432

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTL-Bag2-gRNA2

    Plasmid

    #196252

    Purpose

    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTL-Bag1-gRNA1

    Plasmid

    #196251

    Purpose

    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TIA1 RRM-CFP-FRBx5

    Plasmid

    #103780

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    TIA1 RRM-CFP-FRBx5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. minitalin (headR13DD)

    Plasmid

    #182994

    Purpose

    expression of minitalin wt in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    minitalin (Tln1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBCV

    Plasmid

    #62659

    Purpose

    Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    FLPo
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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