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Omega 1

Plasmid

#118046

Purpose

GoldenBraid2.0 Omega 1 destination vector

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

Plasmid

#197067

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

Depositor

Julia Mahamid

Publication

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MitoTrap-mCherry

Use

CRISPR

Expression

Mammalian

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-R-diLACCO1

Plasmid

#208102

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Publication

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-R-iLACCO1.1

Plasmid

#208103

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Publication

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

AAV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsFastLime

Plasmid

#205754

Purpose

Expression of rsFastLime in mamalian cells

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsFastLime

Expression

Mammalian

Mutation

wt

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-Skylan62A

Plasmid

#205755

Purpose

Expression of Skylan62A in mamalian cells

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

Skylan62A

Expression

Mammalian

Mutation

wt

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-Dronpa

Plasmid

#205756

Purpose

Expression of Dronpa in mammalian cells

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

Dronpa

Expression

Mammalian

Mutation

wt

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1a

Plasmid

#205304

Purpose

Expression of WHaloCaMP1a in E. coli

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1a

Expression

Bacterial

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BioBrick pET-24a(+)

Plasmid

#117802

Purpose

BioBrick pET vector for protein overexpression in E. coli

Depositor

Anthony Forster

Publication

Du et al Biotechnol Bioeng. 2009 Dec 15;104(6):11

Type

Empty backbone

Use

Escherichia coli

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2

Plasmid

#88916

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDsRed-TSTEP_v2

Plasmid

#72336

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Publication

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBbE1k-2

Plasmid

#59945

Purpose

dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

Depositor

Taek Soon Lee

Publication

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Inserts

dihydropterine reductase
pterin-4a-carbinolamine dehydratase

Promoter

pTrc

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pONL-C1_Rab11a

Plasmid

#65707

Purpose

Expresses ONL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_nLightG2-control

Plasmid

#221674

Purpose

Expresses nLightG2-control in neurons

Depositor

Tommaso Patriarchi

Publication

Rohner et al Nat Methods. 2026 Mar;23(3):636-652. doi

Insert

nLightG2-control

Use

AAV

Expression

Mammalian

Promoter

human Synapsin-1

Available since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-WHaloCaMP1a

Plasmid

#205310

Purpose

Production of AAV particles encoding WHaloCaMP1a from the CaMKII promoter

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1a

Use

AAV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSGH2

Plasmid

#186706

Purpose

an artificial heat-inducible promoter

Depositor

Baubak Bajoghli, Thomas Czerny

Publication

Bajoghli et al Dev Biol. 2004 Jul 15;271(2):416-30. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

GFP
luc

Available since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE496

Plasmid

#153248

Purpose

M6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE331

Plasmid

#153240

Purpose

M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE495

Plasmid

#153247

Purpose

M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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