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Plasmid#213207PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHRM1 (CHRM1 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHRM2-DuET
Plasmid#213208PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHRM2 (CHRM2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-hSNCA-NE
Plasmid#215486PurposeLentiviral expression of human alpha-synuclein in mammalian cellsDepositorAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_CRF1.0
Plasmid#208661PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
eTd-CBEm
Plasmid#196602PurposeMammalian expression of eTd-CBEmDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
Tags6*HisExpressionMammalianAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-NLS-mCherry
Plasmid#193762PurposeDoxycycline-inducible (TetOn) mCherry with SV40 NLS, control constructDepositorInsertNLS-mCherry
UseLentiviralTagsmCherryMutationSV40 NLS fused to mCherryPromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_128
Plasmid#160651PurposeExpresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e3 (APOE Human)
TagsKar2 signal sequenceExpressionYeastMutatione3 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-mPGK-eGFP (beta-globin intron)
Plasmid#162513PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intronDepositorInserteGFP
UseAAVExpressionMammalianPromotermPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT447
Plasmid#139387PurposeBile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
UPB-colon (UPB4)
Plasmid#180786PurposeUnique Projection Barcode (UPB4)-Colon for Projection-seqDepositorInserthM3Dq ( partial )
UseAAVExpressionMammalianPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-WW-CIB1-biLINuS
Plasmid#171757PurposeLentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuSDepositorInsertSynthetic gene containing LexA-WW-CIB1-biLINuS
UseLentiviralExpressionMammalianPromoterPGKAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK2020
Plasmid#171932Purposeexpress MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operonDepositorInsertPmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
Tagssuperfolder GFP, mCherryExpressionWormPromoter-Available sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3xVHH05-DsRed
Plasmid#171581PurposeFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorPublicationInsert3xVHH05 (UBE2J1 Human)
ExpressionInsectAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH1-PNGT#2-mCherry
Plasmid#164099PurposeFluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.DepositorInsertPNGT#2-mCherry
UseLentiviral and Synthetic BiologyAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWKS11
Plasmid#139418PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.DepositorInsertmNeonGreen
ExpressionYeastPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRGEB-VirD2-Cas9
Plasmid#139803PurposeExpressing VirD-Cas9 fusion under ubiquitin promoterDepositorInsertVirD2-Cas9
TagsVirD2-Cas9ExpressionPlantPromoterUbiquitinAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R270
Plasmid#138219PurposeExpresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVAX_CBI_AcrIIA4
Plasmid#136662PurposeExpresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAcrIIA4
UseSynthetic BiologyTagsNo tag, No NLSExpressionMammalianPromoterCMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishPromoterUASAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only