We narrowed to 11,797 results for: ARIA;

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pCMV-dCas12a-ABE7

Plasmid

#193644

Purpose

Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA-TadA*7.10, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

Plasmid

#192684

Purpose

Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-RnKIF5C(1-560)-Halo-Flag

Plasmid

#188071

Purpose

Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

Depositor

Kristen Verhey

Article

Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

Insert

rat KIF5C (Kif5c Rat)

Tags

Flag and Halo

Expression

Mammalian

Mutation

aa1-560

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G175R)-Strep

Plasmid

#196211

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G175R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G189E)-Strep

Plasmid

#196212

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G189E

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

Plasmid

#139990

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

VQR=D1135V/R1335Q/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.S72A

Plasmid

#106176

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

hSynapsin

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e-V106W

Plasmid

#193646

Purpose

Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Expression

Mammalian

Mutation

TadA*8e-V106W

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.A184V

Plasmid

#106175

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

hSynapsin

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184V

Plasmid

#106199

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

Plasmid

#139991

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

VRER=D1135V/G1218R/R1335E/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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AT-U1

Plasmid

#112059

Purpose

U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

U1 (RNU1-1 Human)

Expression

Mammalian

Mutation

1 copy of Riboglow tag (truncated variant A) near…

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2

Plasmid

#169463

Purpose

Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.1.7

Plasmid

#169459

Purpose

Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

Plasmid

#139995

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

HF1=N497A/R661A/Q695A/Q926A

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PPARG

Plasmid

#192912

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

PPARG (PPARG Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-WT1

Plasmid

#192906

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of WT1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

WT1 (WT1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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