Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 22,852 results for: Tes

Showing: 7121 - 7140 of 22852 results

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pAG415Gal_Gcn4 WT 44merGFP

Plasmid

#233000

Purpose

Galactose iduced expression of Gcn4 WT 44merGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 30mer

Plasmid

#232986

Purpose

Galactose iduced expression of Gcn4 WT 30mer in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 30mer

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 StoL

Plasmid

#231882

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 StoL

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104L, S117L, S136L, S144L

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 S/ArotoL

Plasmid

#231880

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 L113A+

Plasmid

#231873

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L113A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E2K2

Plasmid

#231868

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E2K2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E2K2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104K, S117E, S136E, S144K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 9acidA

Plasmid

#231867

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 9acidA

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP DEPP1-mNG

Plasmid

#224556

Purpose

Expresses DEPP1 fused to mNEONGreen

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

DEPP1 (DEPP1 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-E566-GS-LOV2-VPR

Plasmid

#231576

Purpose

Potent light-deactivated CRISPRa

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

dSpyCas9-VPR with an AsLOV2 insertion behind E566

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Aro3+

Plasmid

#231884

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro3+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Aro2+

Plasmid

#231883

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WTSLFD

Plasmid

#231881

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WTSLFD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED W

Plasmid

#231871

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoAS

Plasmid

#231859

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED

Plasmid

#231857

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-L1374

Plasmid

#226278

Purpose

Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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