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pLIC-EcTOP

Plasmid

#224463

Purpose

Expression of recombinant Escherichia coli topoisomerase I

Depositor

Yuk-Ching Tse-Dinh

Article

Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

Insert

Escherichia coli TopA

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ikkb-FLAG WT

Plasmid

#23298

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

Ikkbeta (IKBKB Human)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

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SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

Plasmid

#244204

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SV40 1: pBSSVD2005

Plasmid

#21826

Depositor

David Ron

Insert

SV40 Large T (SV40gp6 simian virus)

Expression

Mammalian

Mutation

likely expresses aa109-708 of the large T antigen

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

Plasmid

#238166

Purpose

Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD25

Plasmid

#231547

Purpose

L-arabinose inducible expression of heterologous genes, pBAD24-based backbone with KanR selective marker

Depositor

Martin Wiedmann

Article

Schumann et al J Microbiol Methods. 2025 Mar 18;232-234

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJPC596 FoMV-AmCyan

Plasmid

#234812

Purpose

To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ePPE

Plasmid

#183095

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GqDREADD-dTomato-Fishell-4

Plasmid

#83897

Purpose

Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM3Dq

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

Plasmid

#196678

Purpose

AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

PKIαmut-P2A-mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

35Sp::hCas9

Use

CRISPR

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pENN236_CRISPRcharm_Kv2

Plasmid

#220839

Purpose

CRISPRcharm Kv2 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv2

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b FAST-PETase

Plasmid

#225760

Purpose

Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

FAST-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock

Plasmid

#47334

Purpose

full length clock in pCMV10 with 3x flag at the N-terminus

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

clock

Tags

3x Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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EYFP-NIK

Plasmid

#111208

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

NIK (MAP3K14 Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mCherry (KmR)

Plasmid

#177207

Purpose

Plasmid with p15A origin for constitutive mCherry expression in E. coli

Depositor

Alexey Merz

Insert

mCherry

Expression

Bacterial

Promoter

Synthetic constitutive promoter

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-jGCaMP8f-WPRE (AAV5)

Viral Prep

#176750-AAV5

Purpose

Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8f-WPRE (#176750). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8f-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

Depositor

Loren Looger

Promoter

CamKIIalpha

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only
pdRBFC-B2-YN

Plasmid

#74721

Purpose

Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

Depositor

Aiming Wang

Article

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

HA and YFP N-terminal half domain

Expression

Plant

Promoter

CaMV 35S

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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