We narrowed to 18,762 results for: STO

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AA163

Plasmid

#216061

Purpose

Fragmid fragment: (2A Selection) Stop only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12c2-noncoding

Plasmid

#120877

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12c2

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2899(X-2 MarkerFree)

Plasmid

#73271

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F0

Plasmid

#184162

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT3

Plasmid

#67120

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT3 (STAT3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pmcsg7-mCRT(WT)

Plasmid

#83505

Purpose

Bacterial expression of Calreticulin

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 ISG15

Plasmid

#67054

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ISG15 (ISG15 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GATA1

Plasmid

#67068

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCfB3020(gRNA X-2)

Plasmid

#73282

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TNF

Plasmid

#67062

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TNF (TNF Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
FLAG HDAC5 S259A

Plasmid

#32214

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala

Promoter

CMV

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
mPapaya1-H2B-C-10

Plasmid

#56401

Purpose

Localization: Nucleus/Histones, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

H2B (H2BC11 Human)

Tags

mPapaya1

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EYFP-Clathrin-19

Plasmid

#56584

Purpose

Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Clathrin (CLTB Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pmirGLO-Smad4-1719

Plasmid

#49383

Purpose

Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

Depositor

Martine Roussel

Article

Murphy et al Cancer Res. 2013 Oct 21.

Insert

Smad family member 4 (SMAD4 Human, Mouse)

Use

Luciferase

Promoter

Human phosphoglycerate kinase (PGK) promoter

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3042(gRNA X-4)

Plasmid

#73284

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-NL3

Plasmid

#169108

Purpose

Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-128

Plasmid

#26308

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-128 (MIR128-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
KTK_251

Plasmid

#180546

Purpose

Entry vector containing mScarlet

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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WZLneo-PPARγ2

Plasmid

#34566

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

PPARγ2 (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only