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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 62,316 results for: cloning

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Showing: 7141 - 7160 of 62316 results

  12. pEMI91

    Plasmid
    #74888
    Purpose
    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information
    Depositor
    Piotr Marszalek
    Article
    Scholl et al Biomacromolecules. 2016 Jun 8.
    Insert
    Titin I27 x 9 (TTN Human)
    Tags
    His-tag and Strep-tag II
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74888-plasmid-map-sequence-id-141569

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  13. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid
    #223952
    Purpose
    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223952-plasmid-map-sequence-id-450834

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  14. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138143
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138143-plasmid-map-sequence-id-275400

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Showing: 7141 - 7160 of 62316 results