We narrowed to 11,947 results for: emb
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Plasmid#180358Purposemammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianMutationSEPT9_i1 I281D, M288DPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
Plasmid#128278PurposeExpresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell cultureDepositorInserttwinstar (tsr) (tsr Fly)
Tags6X Histidine tagExpressionBacterialMutationMet1 changed to Pro1; Cys77 silent basepair mutat…PromoterT7Available sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)
Plasmid#87203PurposeTo express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUBL4A (UBL4A Human)
TagsFLAG and GFPExpressionMammalianMutationResidues from 108-130 are inserted.PromoterCMVAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1CFP*
Plasmid#41666DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
TagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-LMNB1
Plasmid#250908PurposeDual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell linesDepositorTagsEGFP on LMNB1 and mKate2-3xNLS on ArfGAP1ExpressionMammalianMutationARFGAP1 amino acids 192–304 onlyPromoterCMVAvailable sinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
B9-COMP-blac-flag-his
Plasmid#111020PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsert6-cysteine protein (B9) (PF3D7_0317100 )
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CPN60-COMP-blac-flag-his
Plasmid#111004PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertCPN60 (PF3D7_1232100 Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHT1-COMP-blac-flag-his
Plasmid#110995PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertchitinase (CHT1) (PF3D7_1252200 )
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1350600-COMP-blac-flag-his
Plasmid#110968PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertPF3D7_1350600 (PF3D7_1350600 )
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
GPR180-COMP-blac-flag-his
Plasmid#110955PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertPF3D7_1213500 (PF3D7_1213500 )
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-577-CFP-578CT
Plasmid#41682DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
TagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)mGAT1-577-YFP-578CT
Plasmid#41681DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
TagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; YFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1-nAChr-beta3-CFP
Plasmid#50485Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
TagsCFPExpressionMammalianMutationCFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3
Plasmid#198914PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCav2.2 (CACNA1B Human)
TagsN-terminal GFP, internal double HAExpressionMammalianMutationexon 18a deleted, exon 19 starts 3 nucleotides ea…PromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0910300-COMP-blac-flag-his
Plasmid#110959PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein, unknown function (PF3D7_0910300 Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1242000-COMP-blac-flag-his
Plasmid#111025PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_1242000 Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
POFUT2-COMP-blac-flag-his
Plasmid#111024PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertGDP fucose protein O-fucosyltransferase 2 (POFUT2) (PFI0445c Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0624400-COMP-blac-flag-his
Plasmid#111022PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_0624400 Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_0107300-COMP-blac-flag-his
Plasmid#111021PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_0107300 Synthetic)
TagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only