We narrowed to 21,681 results for: mpl

1. p2XStrep-ATIC

   Plasmid

   #125685

   Purpose

   expresses human ATIC protein with an N-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   ATIC (ATIC Human)
   

   Use

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

   Plasmid

   #157716

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pD649-HAsp-SEMA3C-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156961

   Purpose

   Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3C (SEMA3C Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RP1B FUS 1-163 QQ4xSS #1

   Plasmid

   #127194

   Purpose

   Protein expression for affinity purification

   Depositor

   Nicolas Fawzi

   Article

   Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

   Insert

   FUS 1-163 QQ4xSS #1 (FUS Human)
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL40C_PGKintron_Cas9_Green

   Plasmid

   #134966

   Purpose

   Lentiviral vector coding Cas9 and mNeonGreen

   Depositor

   Beat Bornhauser

   Article

   Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

   Insert

   Cas9-P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK promoter with beta-globin intron

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR4-hDND1

   Plasmid

   #70070

   Purpose

   Gateway entry vector for human DND1 with stop codon

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGex-4T-2_GEC1

   Plasmid

   #73945

   Purpose

   For recombinant expression of human GEC1/GABARAPL1 in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   GEC1 (GABARAPL1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pEBTet-SNAP-Cep290

   Plasmid

   #136804

   Purpose

   Mammalian expression of the centrosomal protein Cep290 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep290 (CEP290 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Elf5 gRNA

   Plasmid

   #128836

   Purpose

   gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   mElf5 gRNA (Elf5 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-Med25-VBD-6His

    Plasmid

    #64773

    Purpose

    Bacterial expression of His-tagged Med25-VBD

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VBD (MED25 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insert is 394-543 only

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

    Plasmid

    #32008

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    Lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

    Promoter

    CMV, SP6

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Brg1 Del2 pET28a

    Plasmid

    #122116

    Purpose

    Expression Brg1 amino acids 1-837 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    6XHis and His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

    Plasmid

    #111387

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVCT-Dnd1-EGFP

    Plasmid

    #106343

    Purpose

    Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1-EGFP (Dnd1 Synthetic, Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSICO-CPSF6-358-iRFP670

    Plasmid

    #110694

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Truncation of CPSF6 isoform at amino acid 358

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-TetO-Klf5

    Plasmid

    #128830

    Purpose

    dox-dependent expression of mouse Klf5

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-CEACAM5-COMP5AP-AviTag-9xHis

    Plasmid

    #157501

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CEACAM5 (CEACAM5 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

    Plasmid

    #127867

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Human, Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    constructed by blunt end fusion of the 5’ 51 nucl…

    Promoter

    hSyn

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD649-HAsp-KIR3DX1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156774

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR3DX1 (KIR3DX1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid

    #157702

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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