We narrowed to 22,672 results for: LEA
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Plasmid#167296PurposePlasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFPDepositorInsertBAX (BAX Human)
ExpressionMammalianAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJY3948
Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
Tags6XHisExpressionYeastMutationPlease see depositor commentsAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Met1 diUb (1-152)
Plasmid#110766PurposeBacterial expression of Met1 diUbDepositorAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-H29A
Plasmid#80602PurposeExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-H29A
UseAAVExpressionMammalianMutationHis29 mutated to AlaPromoterCBAAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSYC-102
Plasmid#74790PurposepCS4+-NLS-EGFP-T2A-mCherry-CAAXDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-EGFP-T2A-mCherry-CAAX
Available sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Reb_81 aka PAS622
Plasmid#71227PurposeReb_1 with RebB(T28A, D72G)DepositorPublicationInsertReb locus
TagsnoneExpressionBacterialMutationRebB(T28A, D72G), RebA(G53A)Available sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFM032
Plasmid#40076DepositorTypeEmpty backboneTagsDendra2 PAFP, S-peptide, and TEV protease cleavag…ExpressionWormPromoterpie-1Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
ExpressionBacterialPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertECFP-FHA1-JNKsubDD-Citrine-NES
Tags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ-mNeongreen
Plasmid#182392PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusionDepositorInsertVNp-LZ-mNeongreen
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28b_RanWT
Plasmid#186270PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
TagsHis taggedExpressionBacterialPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.HA_mCherry-NLS
Plasmid#178209PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.HA and Nuclear Localization SignalPromotereF1aAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC153
Plasmid#118901Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
TagsTwinStrep, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-YedK
Plasmid#86884PurposeBacterial expression of E. coli SRAP ortholog YedKDepositorAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-373
Plasmid#91779PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP3-373
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1V5-His Snk/Plk2 T239A
Plasmid#22219DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSnk/Plk2 (PLK2 Human)
ExpressionMammalianMutationsingle amino acid substitution mutant, threonine …Available sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239D
Plasmid#22220DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSnk/Plk2 (PLK2 Human)
ExpressionMammalianMutationsingle amino acid substitution mutant, threonine …Available sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJL057
Plasmid#177169PurposeExpresses a nuclease dead MRE11 in human cellsDepositorInsertMRE11 H129N (MRE11 Human)
UseLentiviralExpressionMammalianMutationnuclease-inactivating mutation (H129N)PromoterEF1aAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSin-GFP-Fg
Plasmid#174307PurposeLentiviral expression of GFPDepositorAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by