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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,130 results for: LEA

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Showing: 7161 - 7180 of 12130 results

  1. PHF13 (3O7A)

    Plasmid
    #28155
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PHD finger protein 13 (PHF13 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    PHD-type zinc finger domain (residues 250-301.
    Available Since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. RFXANK (3V3O)

    Plasmid
    #36905
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK (RFXANK Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    amino acid residues 90-260 (ANK repeats)
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. MLH1

    Plasmid
    #32859
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1 (MLH1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    contains amino acids 486-751
    Available Since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. PHF13 (3O70)

    Plasmid
    #28154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PHD finger protein 13 (PHF13 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    PHD-type zinc finger domain (residues 232-281).
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220608
    Purpose
    Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220608-plasmid-map-sequence-id-441203

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  14. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220614
    Purpose
    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220614-plasmid-map-sequence-id-441114

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  18. pCEP4-myc-S (B.1.351 lineage / South African variant)

    Plasmid
    #169849
    Purpose
    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag
    Depositor
    Erik Procko
    Article
    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d
    Insert
    Protein S
    Tags
    HA leader and c-myc tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    169849-plasmid-map-sequence-id-329351

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  19. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  20. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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Showing: 7161 - 7180 of 12130 results