We narrowed to 16,422 results for: LEA

Showing: 7161 - 7180 of 16422 results

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5_T7-GAP43-mScarlet3

Plasmid

#225932

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

GAP43-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_His6_GluA2

Plasmid

#170453

Purpose

Expresses histidine tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Hexa-histidine

Expression

Mammalian

Promoter

Synapsin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-C (TRP1)

Plasmid

#177795

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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MSP2440

Plasmid

#72250

Purpose

Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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4_T7-mScarlet3-RitC

Plasmid

#225931

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3-RitC

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-DroshaS300/302A

Plasmid

#62530

Purpose

Serines 300 and 302 of Drosha are mutated to alanines

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serines 300 and 302 changed alanines (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serines 300 and 302 to alanines

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pB-Tet-On-Arl13b-RhoA sensor

Plasmid

#189613

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

Arl13b

Tags

GFP and mScarlet

Expression

Mammalian

Promoter

Tet3G

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen

Plasmid

#169227

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-GRAB-HTR6-cilia

Plasmid

#189615

Purpose

Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6_GRAB

Use

AAV

Promoter

Syn1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

Plasmid

#163562

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff EYFP

Plasmid

#55651

Purpose

Cre-on/Flp-off EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre on/flp off eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

Plasmid

#163554

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-Anc689-UdgX-NG-nCas9-RBMX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

Plasmid

#187578

Purpose

Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

WRAP53 (WRAP53 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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B-Sniper SpCas9

Plasmid

#207361

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_mMeCP2-HaloTag

Plasmid

#164062

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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