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pAW32

Plasmid

#110225

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW33

Plasmid

#110226

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW34

Plasmid

#110227

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW41

Plasmid

#110228

Purpose

integration of loxP site a distance upstream of gene start site

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for deletion)

Plasmid

#110814

Purpose

Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

Depositor

Jacob Hanna

Publication

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for flox targeting)

Plasmid

#110815

Purpose

Used with pNTK Gatad2a flox allele targeting construct in mouse cells

Depositor

Jacob Hanna

Publication

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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B. adolescentis mRNA toehold switch sensor

Plasmid

#110710

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Publication

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn2 (CG6812)

Plasmid

#110643

Purpose

Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

Depositor

David Sabatini

Publication

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG6812 (CG6812 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn1-3 (CG11739)

Plasmid

#110642

Purpose

Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

Depositor

David Sabatini

Publication

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG11739 (CG11739 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8

Plasmid

#110222

Purpose

Cre-expression plasmid for cassette exchange

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV500

Plasmid

#110722

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Publication

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_Xho

Plasmid

#110223

Purpose

Cre-expression plasmid for cassette exchange but MCS modified compared to pAW8

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB367

Plasmid

#110277

Purpose

gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Publication

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHEEE_02

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Promoter

T7

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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F. prausnitzii mRNA toehold switch sensor

Plasmid

#110716

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Publication

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

F. prausnitzii species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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C. difficile mRNA toehold switch sensor

Plasmid

#110714

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Publication

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

C. difficile species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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R. hominis mRNA species specific sensor

Plasmid

#110713

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Publication

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

R. hominis species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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E. rectale mRNA toehold switch sensor

Plasmid

#110712

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Publication

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. rectale species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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B. breve mRNA toehold switch sensor

Plasmid

#110711

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Publication

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. breve species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge