We narrowed to 13,552 results for: Ski;

1. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234436

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234452

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234450

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC-C SARS-CoV-2 Omicron spike protein

   Plasmid

   #180778

   Purpose

   Sars-CoV-2 variant protein

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   MAC-C SARS-CoV-2 Omicron spike protein
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   C64W , S371L, H69-V70-, S373P, S375F, G339D, N211…

   Promoter

   Available Since

   April 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. LeGO-iG-CSF2RB(A455D)

   Plasmid

   #125751

   Purpose

   Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

   Depositor

   Dirk Heckl

   Article

   Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

   Insert

   CSF2RB(A455D)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   A455D, codon optimized

   Promoter

   SFFV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234449

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

   Plasmid

   #171099

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Ascl1 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2_PROX1_1+3

   Plasmid

   #121176

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

   Plasmid

   #183704

   Purpose

   AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

   Depositor

   Jonathan Ploski

   Insert

   hM4D(Gi)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

    Plasmid

    #183705

    Purpose

    AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

    Depositor

    Jonathan Ploski

    Insert

    hM3D(Gq)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFcpB-DEST

    Plasmid

    #90098

    Purpose

    Gateway destination vector featuring FcpB promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.

    Depositor

    Philip Weyman

    Article

    Bielinski et al PeerJ Preprints. 2017 5:e2907v1

    Type

    Empty backbone

    Use

    Destination vector for gateway expression

    Tags

    Expression

    Mutation

    Promoter

    FcpB

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSPble

    Plasmid

    #63561

    Purpose

    Bleomycin drug selection marker

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    Bleomycin drug selection marker
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDL444

    Plasmid

    #29337

    Depositor

    John Taylor

    Article

    Lazinski et al J Virol. 1993 May . 67(5):2672-80.

    Insert

    Small form of HDV Delta Antigen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The small delta antigen lacks the final 19 aa whi…

    Promoter

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid

    #121174

    Purpose

    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pH4-DEST

    Plasmid

    #90099

    Purpose

    Gateway destination vector featuring H4 promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.

    Depositor

    Philip Weyman

    Article

    Bielinski et al PeerJ Preprints. 2017 5:e2907v1

    Type

    Empty backbone

    Use

    Gateway destination vector for protein expression…

    Tags

    Expression

    Mutation

    Promoter

    H4

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCVD621

    Plasmid

    #66162

    Purpose

    to make a ctxB probe

    Depositor

    James Kaper

    Article

    Michalski et al Infect Immun. 1993 Oct;61(10):4462-8.

    Insert

    ctxB
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Unknown

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

19. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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